Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019033257
pages 55-63

Effects of Substrates on the Production of Fruiting Bodies and the Bioactive Components by Different Cordyceps militaris Strains (Ascomycetes)

Shu-Xia Tao
College of Plant Protection, Jilin Agricultural University, Xincheng Str. 2888, 130118, Changchun, China
Dan Xue
College of Plant Protection, Jilin Agricultural University, Xincheng Str. 2888, 130118, Changchun, China
Zi-Hui Lu
College of Plant Protection, Jilin Agricultural University, Xincheng Str. 2888, 130118, Changchun, China
Hai-long Huang
College of Animal Science and Technology, Jilin Agricultural University, Xincheng Str. 2888, 130118, Changchun, China

RÉSUMÉ

Cordyceps militaris is a type of food and medicinal species and is widely cultured in Asia. Substrate and strain are important factors for the production of fruiting bodies and bioactive components contents in fruiting bodies of C. militaris. This study aimed to select the excellent strains and suitable substrates by six strains of C. militaris cultivated on rice, wheat, and tussah (Antheraea pernyi) pupae. The results showed that the rice and wheat were suitable for fruiting body formation of strain CM3, with yields of 23.19 and 19.07 g per bottle, and biological efficiency of strain CM3 were 62.26% and 54.48%, respectively, which were significantly higher than other strains. Tussah pupae is suitable for fruiting body formation of strain CM9, with fruiting body length, yield, and biological efficiency of 5.57 cm, 6.80 g per each, and 291.70%, respectively, which were significantly higher than other strains. The content of adenosine in fruiting bodies of strain CM9 cultivated on tussah pupae was 2.62 mg g-1, followed by that of strain CM3 on rice of 2.51 mg g-1. The content of cordycepin in fruiting bodies of strain CM4 cultivated on wheat was 5.68 mg g-1, followed by that of strain CM9 on wheat of 5.41 mg g-1. To improve the product quality and the contents of bioactive components, C. militaris strains and substrates should both be considered, that is, different strains should be appropriate for different substrates.

RÉFÉRENCES

  1. Kim HO, Yun JW. A comparative study on the production of exopolysaccharides between two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis in submerged mycelial cultures. J Appl Microbiol. 2005;99(4):728-38.

  2. Dong JZ, Lei C, Ai XR, Wang Y. Selenium enrichment on Cordyceps militaris link and analysis on its main active components. Appl Biochem Biotechnol. 2012;166:1215-24.

  3. Cunningham KG, Manson W, Spring FS, Hutchinson SA. Cordycepin, a metabolic product isolated from cultures of Cordyceps militaris (Linn.) Link. Nature. 1950;166:949.

  4. Xia YL, Luo FF, Shang YF, Chen PL, Lu YZ, Wang CS. Fungal cordycepin biosynthesis is coupled with the production of the safeguard molecule pentostatin. Cell Chem Biol. 2017;24(12):1479-89.

  5. Das SK, Masuda M, Sakurai A, Sakakibara M. Medicinal uses of the mushroom Cordyceps militaris: current state and prospects. Fitoterapia. 2010;81(8):961-68.

  6. Kim YS, Kim EK, Hwang JW, Han YK, Kim SE, Jeong JH, Moon SH, Jeon BT, Park PJ. Radical scavenging activities of Undaria pinnatifida extracts fermented with Cordyceps militaris mycelia. J Microbiol Biotechnol. 2015;25(6):820-27.

  7. Jung K, Kim IH, Han D. Effect of medicinal plant extracts on forced swimming capacity in mice. J Ethnopharmacol. 2004;93(1):75-81.

  8. Lin YW, Chiang BH. Anti-tumor activity of the fermentation broth of Cordyceps militaris cultured in the medium of Radix astragali. Process Biochem. 2008;43(3):244-50.

  9. Reis FS, Barros L, Calhelha RC, Ciric A, van Griensven LJ, Sokovic M, Ferreira IC. The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and antihuman tumor cell lines properties. Food Chem Toxicol. 2013;62:91-98.

  10. Dong CH, Yang T, Lian T. A comparative study of the antimicrobial, antioxidant, and cytotoxic activities of methanol extracts from fruit bodies and fermented mycelia of caterpillar medicinal mushroom Cordyceps militaris (Ascomycetes). Int J Med Mushrooms. 2014;16(5):485-95.

  11. Shrestha B, Han SK, Sung JM, Sung GH. Fruiting body formation of Cordyceps militaris from multi-ascospore isolates and their single ascospore progeny strains. Mycobiology. 2012;40(2):100-6.

  12. Kang N, Lee HH, Park I, Seo YS. Development of high cordycepin-producing Cordyceps militaris strains. Mycobiology. 2017;45(1):31-38.

  13. Lim L, Lee C, Chang E. Optimization of solid state culture conditions for the production of adenosine, cordycepin, and D-mannitol in fruiting bodies of medicinal caterpillar fungus Cordyceps militaris Link (Ascomycetes). Int J Med Mushrooms. 2012;14(2):181-87.

  14. Lin QY, Long LK, Wu LL, Zhang FL, Wu SL, Zhang WM, Sun XM. Evaluation of different agricultural wastes for the production of fruiting bodies and bioactive components by medicinal mushroom Cordyceps militaris. J Sci Food Agric. 2017;97:3476-80.

  15. Tu YQ, Zhu HL, Zeng W, Chen SJ. Determination of D-mannitol in cultivated Cordyceps sinensis. J Biol. 2012;29(3):45-47.

  16. Zhu HT, Chu YQ, Chen L. Optimization of content determination of anthrone-sulfuric acid method on Bletilla striata polysaccharide by orthogonal design. Chin Hosp Pharm J. 2014;34(20):1714-18.

  17. Shi XQ, Gu YY, Li HX, Guo G, Zhu H, Qiao P, Yu ZC. A comparison on morphological characters and active component contents of aweto cultured on silkworm larva inoculated with different Cordyceps militaris strains. Sci Sericult. 2015;41(1):134-39.

  18. Wang F, Liu Q, Zhang JJ, Liu KB, Li K, Liu GJ, Dong CH. Comparative transcriptome analysis between a spontaneous albino mutant and its sibling strain of Cordyceps militaris in response to light stress. Front Microbiol. 2018;9:01237.

  19. Wu CY, Liang ZC, Tseng CY, Hu SH. Effects of illumination pattern during cultivation of fruiting body and bioactive compound production by the caterpillar medicinal mushroom, Cordyceps militaris (Ascomycetes). Int J Med Mushrooms. 2016;18(7):589-97.

  20. Kim SW, Xu CP, Hwang HJ, Choi JW, Kim CW, Yun JW. Production and characterization of exopolysaccharides from an enthomopathogenic fungus Cordyceps militaris NG3. Biotechnol Progr. 2003;19:428-35.

  21. Park JP, Kim SW, Hwang HJ, Cho YJ, Yun JW. Stimulatory effect of plant oils and fatty acids on the exo-biopolymer production in Cordyceps militaris. Enzyme Microb Tech. 2002;31:250-55.

  22. Liang ZC, Liang CH, Wu CY. Various grain substrates for the production of fruiting bodies and bioactive compounds of the medicinal caterpillar mushroom, Cordyceps militaris (Ascomycetes). Int J Med Mushrooms. 2014;16(6):569-78.

  23. Guo MM, Guo SP, Yang HJ, Bu N, Dong CH. Comparison of major bioactive compounds of the caterpillar medicinal mushroom, Cordyceps militaris (Ascomycetes), fruiting bodies cultured on wheat substrate and pupae. Int J Med Mushrooms. 2016;18(4):327-36.

  24. Fan DD, Wang W, Zhong JJ. Enhancement of cordycepin production in submerged cultures of Cordyceps militaris by addition of ferrous sulfate. Biochem Eng J. 2012;60:30-35.

  25. Hung YP, Wang JJ, Wei BL, Lee CL. Effect of the salts of deep ocean water on the production of cordycepin and adenosine of Cordyceps militaris-fermented product. AMB Expr. 2015;5:53.

  26. Lv Y, Ke HT, Chen XP, Fang XMI, Zhou L, Liu X, Cheng M. Nutritional components and content determination in female and male pupae of Antheraea perny. J Anhui Agr Sci. 2016;44(27):61-64.

  27. Chan JS, Barseghyan GS, Asatiani MD, Wasser SP. Chemical composition and medicinal value of fruiting bodies and submerged cultured mycelia of caterpillar medicinal fungus Cordyceps militaris CBS-132098 (Ascomycetes). Int J Med Mushrooms. 2015;17(7):649-59.


Articles with similar content:

Effect of Selenium on the Nutritional Components of Ganoderma lucidum (W.Curt.:Fr.) Lloyd
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Ji-Yun Tong, Shao-Hua Wang, Jian-Zhang Sun, Lei Zhao, Zheng-Dong Zhao
Optimization of Submerged Fermentation Conditions for Lovastatin Production by the Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 5
Zeki Yildiz, Mustafa Yamac, Burcu Atli
Effects of Illumination Pattern during Cultivation of Fruiting Body and Bioactive Compound Production by the Caterpillar Medicinal Mushroom, Cordyceps militaris (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 7
Chin-Yin Tseng, Shu-Hui Hu, Chiu-Yeh Wu, Zeng-Chin Liang
Submerged Cultivation of Mycelium with High Ergothioneine Content from the Culinary-Medicinal King Oyster Mushroom Pleurotus eryngii (Higher Basidiomycetes) and its Composition
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 2
Shin-Yi Lin, Jeng-Leun Mau, Ling-Yi Huang, Kung-Jui Ho, Chih-Hung Liang
Bioconversion of Plant Raw Materials in Value-Added Products by Lentinus edodes (Berk.) Singer and Pleurotus spp.
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
George G. Songulashvili, Nana Aladashvili, Yitzhak Hadar, Michel Penninckx, Mikheil D. Asatiani, Eka Metreveli, Vladimir I. Elisashvili