RT Journal Article
ID 018054f0150d0d4f
A1 Tajima, Katsuya 
A1 Motoi, Masuro
A1 Motoi, Akitomo 
A1 Yamanaka, Daisuke
A1 Ishibashi, Ken-ichi
A1 Adachi, Yoshiyuki
A1 Ohno, Naohito
T1 Immunoreactivity of the Cold Water Extract of Royal Sun Culinary-Medicinal Mushroom, <i>Agaricus brasiliensis</i> Strain KA21 (Agaricomycetes), Assessed by Immunoglobulin Preparations for Intravenous Injection
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2017
FD 2017-10-16
VO 19
IS 8
SP 745
OP 758
K1 <i>Agaricus brasiliensis</i>
K1 immunoglobulin
K1 laccase
K1 medicinal mushrooms
K1 peroxidase
K1 polyphenol
AB The royal sun medicinal mushroom, <i>Agaricus brasiliensis</i>, is a health food material that helps to improve quality of life. <i>A. brasiliensis</i> has long been used as a tea by extraction with cold and hot water. Our group has been investigating the immunopharmacological activities of the <i>A. brasiliensis</i> KA21 strain, which is cultivated outdoors. We prepared cold water (AgCWE) and hot water (AgHWE) extracts of this strain. AgCWE contained a larger proportion of proteins, including enzymes, and showed a brownish color during the extraction process. By contrast,
chemical and immunochemical analyses revealed that AgHWE contained large amounts of &beta;-1,3-/1,6-glucans. In an
attempt to elucidate the immunochemical characteristics of AgCWE, reactivities to immunoglobulin (Ig) preparations
for intravenous injection were analyzed and compared with standard materials. To characterize brownish high–molecular
weight components, standard phenol compounds such as caffeic acid (CA), <i>trans</i>–ferulic acid (FA), and coumaric
acid (CouA) were polymerized to brownish polymerized polyphenols (PPPs) (i.e., polymerized CA, polymerized FA,
and polymerized CouA) by laccase or peroxidase. The results obtained revealed that intravenous Ig reacted with all
PPPs and PPPs cross-reacted with AgCWE and AgHWE. The isotype of the anti-PPP antibody was found to be IgG1,
in contrast to that of the &beta;-glucan antibody, which was mainly IgG2. These results strongly suggest that <i>A. brasiliensis</i> extracts contain immunoreactive components against various classes of Igs.

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