RT Journal Article ID 01cc847b5039e138 A1 Vetchinkina, Elena A1 Gorshkov, Vladimir A1 Ageeva, Marina A1 Gogolev, Yuri A1 Nikitina, Valentina E. T1 Brown Mycelial Mat as an Essential Morphological Structure of the Shiitake Medicinal Mushroom Lentinus edodes (Agaricomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2017 FD 2017-11-01 VO 19 IS 9 SP 817 OP 827 K1 Basidiomycetes morphogenesis K1 brown mycelial mat K1 lectins K1 Lentinus edodes K1 medicinal mushrooms K1 phenol oxidases K1 ultrastructure AB We show here, to our knowledge for the first time, that the brown mycelial mat of the xylotrophic shiitake medicinal mushroom, Lentinus edodes, not only performs a protective function owing to significant changes in the ultrastructure (thickening of the cell wall, increased density, and pigmentation of the fungal hyphae) but also is a metabolically active stage in the development of the mushroom. The cells of this morphological structure exhibit repeated activation of expression of the genes lcc4, tir, exp1, chi, and exg1, coding for laccase, tyrosinase, a specific transcription factor, chitinase, and glucanase, which are required for fungal growth and morphogenesis. This study revealed the maximum activity of functionally important proteins with phenol oxidase and lectin activities, and the emergence of additional laccases, tyrosinases, and lectins, which are typical of only this stage of morphogenesis and have a regulatory function in the development and formation of fruiting bodies. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,53402a884df3ae15,01cc847b5039e138.html