RT Journal Article ID 0b9b4e527c117a40 A1 Zhou, Xuan-Wei A1 Xie, Minqi A1 Hong, Fan A1 Li, Qi-zhang T1 Genomic Cloning and Characterization of a FIP-gsi Gene Encoding a Fungal Immunomodulatory Protein from Ganoderma sinense Zhao et al. (Aphyllophoromycetideae) JF International Journal of Medicinal Mushrooms JO IJM YR 2009 FD 2009-03-18 VO 11 IS 1 SP 77 OP 86 AB A genomic DNA sequence encoding fungal immunomodulatory protein (FIP), the FIP-gsi gene, was isolated from Ganoderma sinense (Ganodermataceae, Basidiomycetes) using genomic walker technology. Analysis of 1072-bp segments revealed that the gene contained a 501-bp 5'-flanking region, a 333-bp open read frame (ORF), and a 238-bp 3'-flanking region. There is one putative TATA box and one possible CAAT box lieing in the 5'-flanking region. The ORF encodes a 12.4-kDa precursor polypeptide. One intron was found in the 5'-flanking region. The deduced amino acid sequence of FIP-gsi shares high similarity with other FIPs, for example, G. lucidum (FIP-glu), G. japonicum (FIP-gja), G. tsugae (FIP-gts), Flammulina velutipes (FIP-fve), and Volvariella volvacea (FIP-vvo), which were found to be 99%, 86%, 86%, 61%, and 56% identical, respectively. The cloning of the genomic DNA sequence is an important foundation for further study of its structure, expression, and regulatory mechanisms. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,5a77ac4169e8d0a9,0b9b4e527c117a40.html