RT Journal Article
ID 2e112cf41f658398
A1 Minato, Ken-ichiro
A1 Mizuno, Masashi
A1 Kawakami, Sachiko
A1 Tatsuoka, Shigenobu
A1 Denpo, Yutaka
A1 Tokimoto, Keisuke
A1 Tsuchida, Hironobu
T1 Changes in Immunomodulating Activities and Content of Antitumor Polysaccharides During the Growth of Two Medicinal Mushrooms, Lentinus edodes (Berk.) Sing, and Grifola frondosa (Dicks.: Fr.) S. F. Gray
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2001
FD 2001-03-01
VO 3
IS 1
OP 7
AB The immunomodulating effects of the growing medicinal mushrooms Lentinus edodes and Grifola frondosa on tumor necrosis factor-α (TNF-α) and nitric oxide (NO) production from murine peritoneal macro-phages were investigated. The weight of the L. edodes fruiting body in this study increased from 9 g to 18 g during growth, and that of G. frondosa increased from 130 g to 200 g. L edodes increased TNF-α production from approximately 500 pg/ml to 700 pg/ml and NO production from 4 μM to 7 μM. Thereafter, it finally decreased TNF-α levels to 500 pg/ml and NO to 5 μM through the growth periods. The growing G. frondosa increased TNF-α production from approximately 300 pg/ml to 500 pg/ml, which finally decreased to 260 pg/ml. NO production increased from 5 to 7 μM, and finally declined to 4 μM during growth. Moreover, to clarify whether these changes in immunomodulating activities of the two mushrooms resulted from changes in antitumor polysaccharides, we examined the contents of lentinan in L. edodes and glucan in G. frondosa (GGF) in the growing mushrooms, respectively, by enzyme-linked imnuinosorbent assay (ELISA). The content of lentinan increased to 1.5 mg/g fresh weight (f.w.), and thereafter finally decreased to 1.1 mg/g f.w. during growth of L. edodes. The content of GGF increased to 2.7 mg/g f.w., but finally decreased to 1.3 mg/g f.w. during growth of G. frondosa. These results suggested that a suitable period in which to harvest the mushroom at its peak antitumor activity was found.
PB Begell House
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