%0 Journal Article %A Wang, Mingxing %A Zhang, Yanqiu %A Xiao, Xulang %A Xu, Duoduo %A Gao, Yang %A Gao, Qipin %D 2017 %I Begell House %K apoptosis, Hericium erinaceus, medicinal mushrooms, polysaccharide, precancerous human gastric cells %N 12 %P 1053-1060 %R 10.1615/IntJMedMushrooms.2017024975 %T A Polysaccharide Isolated from Mycelia of the Lion’s Mane Medicinal Mushroom Hericium erinaceus (Agaricomycetes) Induced Apoptosis in Precancerous Human Gastric Cells %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2dab30ba797125f4,30181a9427a1f563.html %V 19 %X Hericium erinaceus is typically used in traditional Chinese medicine for mucosal protection, healing of gastric ulcers, and treatment of gastritis. We purified from the cultured mycelia of H. erinaceus a polysaccharide with anti–gastric ulcer and antigastritis activity, but its effects on gastric malignancy have not been elucidated. We examined the differential effects of this purified polysaccharide, named EP-1, on the human gastric (GES-1) cell line and a precancerous cell line (MC) that was transformed from GES-1 using N-methyl-N'-nitro-N-nitrosoguanidine. We observed that the polysaccharide potently induced cell apoptosis and cell cycle arrest at the G0/G1 phase in the MC cell line but did not have any effect on the GES-1 cell line at the same doses. Further mechanistic studies revealed that the polysaccharide exerted its activity through an apoptosis-associated pathway by modulating the expression of Bax, Bcl-2, and caspase-3. Differential effects of the polysaccharide on the GES-1 and MC cell lines indicate that the polysaccharide was effective in preventing gastric cancer progression. %8 2018-02-05