%0 Journal Article
%A Wang, Mingxing
%A Zhang, Yanqiu
%A Xiao, Xulang
%A Xu, Duoduo
%A Gao, Yang
%A Gao, Qipin
%D 2017
%I Begell House
%K apoptosis, Hericium erinaceus, medicinal mushrooms, polysaccharide, precancerous human gastric cells
%N 12
%P 1053-1060
%R 10.1615/IntJMedMushrooms.2017024975
%T A Polysaccharide Isolated from Mycelia of the Lion’s Mane Medicinal Mushroom Hericium erinaceus (Agaricomycetes) Induced Apoptosis in Precancerous Human Gastric Cells
%U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2dab30ba797125f4,30181a9427a1f563.html
%V 19
%X Hericium erinaceus is typically used in traditional Chinese medicine for mucosal protection, healing of gastric ulcers, and treatment of gastritis. We purified from the cultured mycelia of H. erinaceus a polysaccharide with anti–gastric ulcer and antigastritis activity, but its effects on gastric malignancy have not been elucidated. We examined the differential effects of this purified polysaccharide, named EP-1, on the human gastric (GES-1) cell line and a precancerous cell line (MC) that was transformed from GES-1 using N-methyl-N'-nitro-N-nitrosoguanidine. We observed that the polysaccharide potently induced cell apoptosis and cell cycle arrest at the G0/G1 phase in the MC cell line but did not have any effect on the GES-1 cell line at the same doses. Further mechanistic studies revealed that the polysaccharide exerted its activity through an apoptosis-associated pathway by modulating the expression of Bax, Bcl-2, and caspase-3. Differential effects of the polysaccharide on the GES-1 and MC cell lines indicate that the polysaccharide was effective in preventing gastric cancer progression.
%8 2018-02-05