RT Journal Article
ID 31241b126382b416
A1 Porras-Arboleda, Sandra M.
A1 Valdez-Cruz, Norma A.
A1 Rojano, Benjamin
A1 Aguilar, Cecilia
A1 Rocha-Zavaleta, Leticia
A1 Trujillo-Roldan, Mauricio
T1 Mycelial Submerged Culture of New Medicinal Mushroom, Humphreya coffeata (Berk.) Stey. (Aphyllophoromycetideae) for the Production of Valuable Bioactive Metabolites with Cytotoxicity, Genotoxicity, and Antioxidant Activity
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2009
FD 2009-11-16
VO 11
IS 4
SP 335
OP 350
K1 medicinal mushrooms
K1 Humphreya coffeata
K1 submerged culture
K1 exopolysaccharides
K1 genotoxicity
K1 cytotoxicity
K1 antioxidant activity
AB The mycelial submerged culture of the Colombian mushroom Humphreya coffeata, the production of exopolysaccharides (EPS), and the evaluation of the supernatant components in terms of bioactivity were investigated. Three carbon sources, three concentrations of carbon and nitrogen sources, and three pH values were evaluated. The optimal source was lactose (over sucrose and glucose), and the maximal biomass and EPS concentrations (15.5 g/L and 6.9 g/L, respectively) were obtained using lactose at 50 g/L, yeast extract 10 g/L, and an initial pH of 4.5. The cytotoxic, proliferative, genotoxic, and antioxidant activities were evaluated in lyophilized culture filtrates. Cytotoxicity was analyzed on human nontumorigenic keratinocytes (HaCaT), two human epithelial cell lines of cervical cancer (HeLa and InBl), and one lymphoma cell line (Jurkat). None of the filtrate concentrations that showed a cytotoxic effect on Jurkat cells (250-2500 μg/mL) were toxic for control HaCat cells and cervical cancer (HeLa and InBl). Furthermore, DNA damage was observed at a high extract concentration (2500 μg/mL), and genoprotection was observed using the lowest concentration tested (250 μg/mL). In addition, antioxidant activity was observed via the superoxide anion and the DPPH scavenging. This suggests that H. coffeata extracts can repress initiation and/or break the chain of oxidation reactions.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,4554be083929b23c,31241b126382b416.html