RT Journal Article ID 434ca0eb789d90ca A1 Jaszek, Magdalena A1 Osinska-Jaroszuk, Monika A1 Sulej, Justyna A1 Matuszewska, Anna A1 Stefaniuk, Dawid A1 Maciag, Kamil A1 Polak, Jolanta A1 Matuszewski, Lukasz A1 Grzywnowicz, Krzysztof T1 Stimulation of the Antioxidative and Antimicrobial Potential of the Blood Red Bracket Mushroom Pycnoporus sanguineus (Higher Basidiomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2015 FD 2015-11-09 VO 17 IS 8 SP 701 OP 712 K1 medicinal mushrooms and fungi K1 low-molecular-weight subfraction K1 antioxidants K1 antimicrobial properties K1 Pycnoporus sanguineus K1 idiophasic cultures K1 SEM AB The antioxidative and antibacterial properties of low-molecular-weight secondary metabolite subfractions (ex-LMS) from cultures of Pycnoporus sanguineus cultivated under different temperature conditions (25°C [ex-LMSa] and 30°C [ex-LMSb]) were assessed. The antioxidative properties were studied using chemiluminometric measurement, an ABTS assay, and a DPPH reduction rate assay with Trolox and ascorbic acid as the control. The values noted for the ex-LMSb were significantly higher than those for ex-LMSa: 97%, 52%, and 31% for chemiluminometric measurement, the ABTS assay, and the DPPH assay, respectively, at a concentration of 50 µg/mL. Half-maximal effective concentrations reached 4.17 µg/mL for chemiluminometric measurement, 47.25 µg/mL for the ABTS assay, and 51.46 µg/mL for DPPH assay. Toxicity tests against Vibrio fischeri yielded 99.8% for ex-LMSa and 99.85% for ex-LMSb. Antibacterial activity toward Staphylococcus aureus was observed in the ex-LMSb fractions (inhibition zone, 23.5 mm; minimum inhibitory concentration, 0.12 mg/mL). Scanning electron microscopy images exhibited severe disruption of the bacterial cells treated with ex-LMSb compared with the control. The results obtained suggest that the extracellular fluid isolated from P. sanguineus−submerged cultures might be a good source of antioxidative and antibacterial compounds. In addition, the increase in the culture temperature evidently enhanced the bioactive properties of the preparation. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,5c93eb0962bab6ac,434ca0eb789d90ca.html