RT Journal Article
ID 6860fefb6b964bdd
A1 Hsu, Keng-Hao
A1 Lee, Yi-Ru
A1 Lin, Yan-Liang
A1 Chu, Fang-Hua
T1 Cytochrome P450 Genes in Medicinal Mushroom Antrodia cinnamomea T.T. Chang et W.N. Chou (Higher Basidiomycetes) are Strongly Expressed During Fruiting Body Formation
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2011
FD 2011-12-15
VO 13
IS 6
SP 513
OP 523
K1 medicinal mushrooms
K1 Antrodia cinnamomea
K1 cytochrome P450
K1 fruiting body
AB Medicinal mushroom Antrodia cinnamomea is a higher Basidiomycetes endemic to Taiwan, where it is commonly used as a traditional folk medicine. It is well known for its multiple biologic activities and its potential for commercial development. Here, ten full lengths of cytochrome P450 (CYP) genes (ac-1 to ac-10) from A. cinnamomea were cloned and identified. With the exception of ac-3 and ac-8, which will probably be assigned as new CYP families, these genes had more than 40% amino acid identity and close evolutionary relationships to known CYPs. Among the ten genes, only Ac-7 did not possess a transmembrane domain but had an N-terminal signal peptide, so it was considered a novel extracellular CYP. The ten A. cinnamomea CYPs had different expression profiles in different growth conditions. In general, they were strongly expressed during the formation of fruiting bodies, especially in natural basidiomycetes. The expression of six CYPs of A. cinnamomea (ac-1 to ac-3 and ac-5 to ac-7) were strictly inhibited in the mycelia cell type. It was therefore concluded that these CYPs are most active in the fruiting bodies of A. cinnamomea.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7b5e519241ca8193,6860fefb6b964bdd.html