RT Journal Article ID 6860fefb6b964bdd A1 Hsu, Keng-Hao A1 Lee, Yi-Ru A1 Lin, Yan-Liang A1 Chu, Fang-Hua T1 Cytochrome P450 Genes in Medicinal Mushroom Antrodia cinnamomea T.T. Chang et W.N. Chou (Higher Basidiomycetes) are Strongly Expressed During Fruiting Body Formation JF International Journal of Medicinal Mushrooms JO IJM YR 2011 FD 2011-12-15 VO 13 IS 6 SP 513 OP 523 K1 medicinal mushrooms K1 Antrodia cinnamomea K1 cytochrome P450 K1 fruiting body AB Medicinal mushroom Antrodia cinnamomea is a higher Basidiomycetes endemic to Taiwan, where it is commonly used as a traditional folk medicine. It is well known for its multiple biologic activities and its potential for commercial development. Here, ten full lengths of cytochrome P450 (CYP) genes (ac-1 to ac-10) from A. cinnamomea were cloned and identified. With the exception of ac-3 and ac-8, which will probably be assigned as new CYP families, these genes had more than 40% amino acid identity and close evolutionary relationships to known CYPs. Among the ten genes, only Ac-7 did not possess a transmembrane domain but had an N-terminal signal peptide, so it was considered a novel extracellular CYP. The ten A. cinnamomea CYPs had different expression profiles in different growth conditions. In general, they were strongly expressed during the formation of fruiting bodies, especially in natural basidiomycetes. The expression of six CYPs of A. cinnamomea (ac-1 to ac-3 and ac-5 to ac-7) were strictly inhibited in the mycelia cell type. It was therefore concluded that these CYPs are most active in the fruiting bodies of A. cinnamomea. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7b5e519241ca8193,6860fefb6b964bdd.html