RT Journal Article ID 72a19aba05908d62 A1 Song, Wei A1 Van Griensven, Leonardus Johannes Lambertus Donatus A1 Shnyreva, Alla V. T1 Extracts of Medicinal Mushrooms Agaricus bisporus and Phellinus linteus Induce Proapoptotic Effects in the Human Leukemia Cell Line K562 JF International Journal of Medicinal Mushrooms JO IJM YR 2010 FD 2010-05-26 VO 12 IS 2 SP 167 OP 175 K1 medicinal mushrooms K1 Agaricus bisporus K1 Phellinus linteus K1 mushroom ethanol extracts K1 proapoptotic and anticancer activities AB We have studied the effects of Agaricus bisporus and Phellinus linteus ethanol extracts on transcriptional regulation of genes involved in cytokine release and apoptosis in the human leukemia cell line K562. In particular, we applied quantitative real-time PCR (Q-PCR) assays to monitor alterations of gene expression for the following genes: Bcl-2, Casp-9, NF-κB, TNF-α, IFN-γ, and IL-10. We found stronger proapoptotic activity for the Ph. linteus alcohol extract on K562 cells than for the A. bisporus extract: 4.4- and 2.2-fold increase of Bcl-2 and Casp-9 transcripts. Mushroom alcohol extracts are suggested to exert their effects on tumor cells via the induction of apoptosis. K562 leukemia cells were shown to be most responsive to the transcriptional induction of tumor necrosis factor TNF-α when stimulated with IFN-γ and then treated with Ph. linteus alcohol extract: up to a 4.5-fold increase. Treatment of K562 cells with A. bisporus extract promoted transcription of the cytokine gene IL-10. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,40aa21637478b2ab,72a19aba05908d62.html