RT Journal Article ID 7895d53d647838f8 A1 Yang, Hairui A1 Sun, Wenbo A1 Zhang, Jinsong A1 Zhang, Yaping A1 Yang, Yan A1 Wu, Di A1 Liu, Yanfang A1 Qiankun, Zheng A1 Min, Li A1 Wang, Wenhan A1 Jia, Wei T1 Autophagy Inhibition Enhances SPCA-1 Cell Proliferation Inhibition Induced by By-1 from the Stout Camphor Medicinal Mushroom, Taiwanofungus camphoratus (Agaricomycetes) JF International Journal of Medicinal Mushrooms JO IJM YR 2018 FD 2018-05-16 VO 20 IS 4 SP 321 OP 335 K1 apoptosis K1 autophagy K1 By-1 K1 cell proliferation K1 medicinal mushrooms K1 Taiwanofungus camphoratus AB Taiwanofungus camphoratus has been reported to have antitumor effects against various cancer cells. The aim of this study was to investigate the direct inhibitory effect of By-1 (3-isobutyl-l-methoxy-4-[4'-(3-methylbut-2-enyloxy)phenyl]-1H-pyrrole-2,5-dione), a compound from spent broth from submerged cultures of T. camphoratus, on human lung adenocarcinoma cells and to determine the molecular mechanism underlying this effect. The growth-inhibitory assay and colony formation assay showed that cell viability was significantly decreased. A By-1 concentration of 300 μmol/L caused 73.55% cell death and at a concentration of 240 μmol/L led to a 58% reduction in the number of colonies. The wound-healing assay showed that the distance of migration was 0.3 times shorter than that of untreated cells. Flow cytometry revealed that By-1 could suppress DNA synthesis, cause cell cycle arrest at the S phase, and induce apoptosis in a reactive oxygen species-dependent manner. Furthermore, the expression of caspase-3 and P53 was 4 times higher than that in untreated cells, and the antiapoptotic protein Bcl-2 was decreased 2 times compared with the protein in untreated cells. It is interesting to note that apoptosis and autophagy were both induced during treatment with By-1, and autophagy inhibition decreased cell proliferation. By-1 potently inhibited the growth of SPCA-1 cells by inducing cell cycle arrest and apoptosis. The combination of proapoptosis agents and antiautophagy agents could effectively enhance anticancer efficacy, which may be a new strategy in treating non-small cell lung cancer. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,31a39db86881b79c,7895d53d647838f8.html