%0 Journal Article %A Maiti, Swatilekha %A Mallick, Sanjaya Kumar %A Bhutia, Sujit Kumar %A Behera, Birendra %A Mandal, Mohitosh %A Maiti, Tapas K %D 2011 %I Begell House %K medicinal mushrooms, Pleurotus ostreatus, antitumor protein, Dalton’s lymphoma, immunomodulation %N 5 %P 427-440 %R 10.1615/IntJMedMushr.v13.i5.20 %T Antitumor Effect of Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Jacq.: Fr.) P. Kumm., Derived Protein Fraction on Tumor-Bearing Mice Models %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,420b184f15148de0,7fdfc12b14fa45a1.html %V 13 %X Previously, we reported the in vitro anticancer and immunomodulatory effect of a protein fraction designated as Cibacron blue affinity purified protein (CBAEP) obtained from the culinary-medicinal oyster mushroom, Pleurotus ostreatus. In the present study, we investigated the in vivo antitumor potential of CBAEP in different tumor-bearing mice models and studied the detailed mechanism of tumor regression in Dalton lymphoma (DL)-bearing mice. The lethal dose (LD50) of CBAEP was found to be 55 mg/kg body weight and sublethal doses (5 mg/kg and 10 mg/kg body weight) showed a prolonged tumor survival time in DL, Sarcoma-180, and B16F0 melanoma tumor-bearing mice. Further, CBAEP reduced about 35.68 and 51.43% DL cell growth in 5 and 10 mg/kg body weight, respectively. The in vivo CBAEP treatment showed an apoptotic feature as demonstrated in morphological study and sub-G0/G1 population in cell cycle and Western blot of DL cells. CBAEP also activated immunosuppression condition in DL tumor-bearing host. It also stimulated immune cells in the presence of nonspecific immunostunulator (LPS and ConA) ex vivo as well as enhanced Th1 response with production of TNF-α, IFN-γ, and IL-2. Moreover, it activated tumor-associated macrophages and NK cells. The present findings revealed the potent antitumor property of CBAEP, which might help in developing a new anticancer drug. %8 2011-11-23