%0 Journal Article
%A Maiti, Swatilekha
%A Mallick, Sanjaya Kumar
%A Bhutia, Sujit Kumar
%A Behera, Birendra
%A Mandal, Mohitosh
%A Maiti, Tapas K
%D 2011
%I Begell House
%K medicinal mushrooms, Pleurotus ostreatus, antitumor protein, Dalton’s lymphoma, immunomodulation
%N 5
%P 427-440
%R 10.1615/IntJMedMushr.v13.i5.20
%T Antitumor Effect of Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Jacq.: Fr.) P. Kumm., Derived Protein Fraction on Tumor-Bearing Mice Models
%U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,420b184f15148de0,7fdfc12b14fa45a1.html
%V 13
%X Previously, we reported the in vitro anticancer and immunomodulatory effect of a protein fraction designated as Cibacron blue affinity purified protein (CBAEP) obtained from the culinary-medicinal oyster mushroom, Pleurotus ostreatus. In the present study, we investigated the in vivo antitumor potential of CBAEP in different tumor-bearing mice models and studied the detailed mechanism of tumor regression in Dalton lymphoma (DL)-bearing mice. The lethal dose (LD50) of CBAEP was found to be 55 mg/kg body weight and sublethal doses (5 mg/kg and 10 mg/kg body weight) showed a prolonged tumor survival time in DL, Sarcoma-180, and B16F0 melanoma tumor-bearing mice. Further, CBAEP reduced about 35.68 and 51.43% DL cell growth in 5 and 10 mg/kg body weight, respectively. The in vivo CBAEP treatment showed an apoptotic feature as demonstrated in morphological study and sub-G0/G1 population in cell cycle and Western blot of DL cells. CBAEP also activated immunosuppression condition in DL tumor-bearing host. It also stimulated immune cells in the presence of nonspecific immunostunulator (LPS and ConA) ex vivo as well as enhanced Th1 response with production of TNF-α, IFN-γ, and IL-2. Moreover, it activated tumor-associated macrophages and NK cells. The present findings revealed the potent antitumor property of CBAEP, which might help in developing a new anticancer drug.
%8 2011-11-23