Published 4 issues per year
ISSN Print: 0731-8898
ISSN Online: 2162-6537
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Attenuation of Leukemia/Lymphoma-Related Factor Protein Expression Inhibits Glioma Cell Proliferation and Invasion
ABSTRACT
Overexpression of leukemia/lymphoma-related factor (LRF), which is an erythroid myeloid ontogenic factor protein, occurs in different cancers, including glioma. LRF is also reported to have an oncogenic activity in various human cancers. This study investigated the effect of LRF knockdown on the regulation of glioma growth. LRF short hairpin RNA (shRNA) suppressed the expression of LRF protein in a glioma cell line (GL261-EGFP) compared to the negative control vector−transfected glioma cells. LRF knockdown also reduced glioma cell viability and enhanced cisplatin-induced apoptosis in glioma cells. AKT activation and the expression of various cell cycle−related genes were inhibited following LRF knockdown. The effect on growth and migration is related to dose response results of AKT and nuclear factor-kappa B (NF-κB) inhibitors. These data demonstrate that LRF may play a role in glioma progression, suggesting that inhibition of LRF expression using LRF shRNA should be further evaluated as a novel target for the control of glioma.
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Zhu Min, Wang Peng, Feng Fan, Li Ming-Yang, LRF inhibits p53 expression in colon cancer cells via modulating DAP5 activity, Cell Biochemistry and Function, 35, 7, 2017. Crossref
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Constantinou Caterina, Spella Magda, Chondrou Vasiliki, Patrinos George P., Papachatzopoulou Adamantia, Sgourou Argyro, The multi-faceted functioning portrait of LRF/ZBTB7A, Human Genomics, 13, 1, 2019. Crossref