Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
Journal of Environmental Pathology, Toxicology and Oncology
IF: 1.625 5-Year IF: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2014008425
pages 83-98

Protective Effect of Acacia ferruginea against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways

Kunnathur Murugesan Sakthivel
Karunya University, Department of Biotechnology, Karunya Nagar, Coimbatore−641114, Tamil Nadu, India; Laboratory of Cytogenetics and Molecular Diagnostics, Regional Cancer Centre, Medical College Post, Trivandrum 695011, Kerala, India
Chandrasekaran Guruvayoorappan
Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram - 695011, Kerala, India

ABSTRACT

In the present study, we evaluated the protective effect of A. ferruginea extract against ulcerative colitis (UC). Male Wistar rats received A. ferruginea extract (10 mg/kg body weight) or sulfasalazine (100 mg/kg body weight) for 5 consecutive days before inducing UC via intrarectal acetic acid (3%) administration. Colonic mucosal injury was assessed by macroscopic scoring, vascular permeability testing, and histopathological examination. The mucosal contents of glutathione, lipid peroxidation, superoxide dismutase, and nitric oxide were evaluated as parameters for the redox state. Inflammatory response was determined by measuring inducible nitric oxide synthase (iNOS) and cyclo-oxygenase (COX-2) expression. Myeloperoxidase (MPO), lactate dehydrogenase assay (LDH), tumor necrosis factor (TNF-α), and interleukins (IL-1β and IL-6) were measured using ELISA. Transcription factor profiling of nuclear factor (NF)-κB subunits (p65/p50) was also conducted using ELISA. All of the relevant parameters were altered in rats with UC, and these parameters improved in animals that received A. ferruginea extract. Colonic mucosal injury parallels antioxidant and anti-inflammatory evaluations, and A. ferruginea extract was considered comparable to the standard treatment drug sulfasalazine. Histopathological studies confirmed these findings. A. ferruginea extract inhibited the activation and translocation of transcription factors, that is, NF-κB subunits (p65/p50). The results of our investigation clearly indicate that treatment with A. ferruginea extract exerted a marked protective effect against experimental UC via modulation of oxidant/anti-oxidant balance and inhibition of inflammatory mediators.


Articles with similar content:

Protective Role of Naringenin Against Doxorubicin-Induced Cardiotoxicity in a Rat Model: Histopathology and mRNA Expression Profile Studies
Journal of Environmental Pathology, Toxicology and Oncology, Vol.33, 2014, issue 4
Kumaresan Ganesan, Murugesan Ramachandran, Swathika Subburaman
Anti-Inflammatory Effects of Ethanol Extract of Lion’s Mane Medicinal Mushroom, Hericium erinaceus (Agaricomycetes), in Mice with Ulcerative Colitis
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 3
Hong-Yu Xu, Xin Xu, Zhenming Lu, Yan Geng, Mingming Qin, Zheng-Hong Xu, Jin-Song Shi
Protective Effect of Curcumin Against Carbofuran-Induced Toxicity in Wistar Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.36, 2017, issue 1
Binitha P. Purushothaman, Ramadasan Kuttan
Tomentosin Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Inflammatory Response by Suppression of the NF-κB Pathway in a Mouse Model of Sepsis
Journal of Environmental Pathology, Toxicology and Oncology, Vol.39, 2020, issue 4
Jian Wan, Jie Sun, Conghui Fan, Yongyi Wang, Haijun Zhu
Protective Effect of Solanum muricatum on Tumor Metastasis by Regulating Inflammatory Mediators and Nuclear Factor-Kappa B Subunits
Journal of Environmental Pathology, Toxicology and Oncology, Vol.34, 2015, issue 3
Chandrasekaran Guruvayoorappan, Kittappa Shathish, Kunnathur Murugesan Sakthivel