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Journal of Environmental Pathology, Toxicology and Oncology
IF: 1.15 5-Year IF: 1.4 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v30.i3.70
pages 241-249

Effect of Benzo[a]pyrene on the Production of Vascular Endothelial Growth Factor by Human Eosinophilic Leukemia EoL-1 Cells

Jie Gu
Department of Biology, Hong Kong Baptist University, Hong Kong
Lai-Sheung Chan
Department of Biology, Hong Kong Baptist University, Hong Kong
Chris Kong-Chu Wong
Department of Biology, Hong Kong Baptist University, Hong Kong
Ngok-Shun Wong
Department of Biology, Hong Kong Baptist University, Hong Kong
Chun-Kwok Wong
Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong
Kok-Nam Leung
School of Life Sciences, The Chinese University of Hong Kong, Hong Kong
Naiki K. Mak
Department of Biology, Hong Kong Baptist University, Hong Kong, People's Republic of China

ABSTRACT

Benzo[a]pyrene (BaP) has been shown to affect both the development and response of T and B cells in the immune system. However, the effect of BaP on other immune cells, such as eosionophils, is unknown. In this study, we investigated the effect of BaP on the production of vascular endothelial growth factor (VEGF) using an in vitro eosinophilic EoL-1 cell and human umbilical vein endothelial cell (HUVEC) co-culture system. EoL-1−conditioned medium was found to promote the growth of HUVEC in a time-dependent manner. The growth stimulating activity was due to the production of VEGF by the EoL-1 cells. The production of VEGF was correlated with the enhanced expression of the phosphorylated form of extracellular signal-regulated kinases (p-ERKs) and the upregulated expression of VEGF mRNA. Furthermore, BaP-induced expression of VEGF mRNA was reduced by the ERK inhibitor PD98059. Results from this study suggested that BaP might affect the growth of endothelial cells through the modulation of VEGF production by eosinophils.

KEY WORDS: BaP, EoL-1, HUVEC, VEGF, co-culture