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Journal of Environmental Pathology, Toxicology and Oncology
IF: 1.15 5-Year IF: 1.4 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v30.i3.20
pages 189-197

Differential Modifying Effects of Food Additive Butylated Hydroxytoluene Toward Radiation and 4-Nitro-quinoline 1-Oxide−Induced Genotoxicity in Yeast

Kshiti B. Anjaria
Radiological Physics and Advisory Division, Bhabha Atomic Research Centre, Mumbai, India
Nagesh N. Bhat
Radiological Physics and Advisory Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India
Kapil B. Shirsath
Radiological Physics and Advisory Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India
B. Sreedevi
Radiological Physics and Advisory Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India

ABSTRACT

The modifying effect of butylated hydroxytoluene (BHT) on 60Co gamma radiation and 4-nitro-quinoline 1-oxide-induced gene conversion and back mutation frequencies was investigated using diploid yeast Saccharomyces cerevisiae D7. Cells were exposed to 100 or 400 Gy in the presence of 0.025−0.25 mM BHT. BHT exhibited radioprotection and significantly reduced radiation-induced gene conversion and back mutation frequencies as well as cell killing. In another set of experiments, cells were simultaneously treated with 0.025−0.1 mM BHT and 0.5 μM 4-NQO. BHT significantly enhanced 4-NQO-induced gene conversion and back mutation frequencies. BHT post-treatment did not modify radiation-induced genetic events but enhanced 4-NQO-induced back mutation frequencies, indicating its potential to act as a tumor-promoting agent with 4-NQO.