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Journal of Environmental Pathology, Toxicology and Oncology

Impact factor: 1.154

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2013007532
pages 241-244

Hydration with Saline Decreases Toxicity of Mice Injected With Calcitriol in Preclinical Studies

Amir A. Azari
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin
Mozhgan R. Kanavi
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin; Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Soesiawati R. Darjatmoko
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin
Vivian Lee
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin
KyungMann Kim
Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, Wisconsin
Heather D. Potter
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin
Daniel M. Albert
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin

ABSTRACT

The effectiveness of saline injection in reducing the toxicity profile of calcitriol when coadministered in mice was evaluated. Mortality was used as an end point to study the toxic effects of calcitriol; the relative risk of mortality in mice injected with saline was evaluated from our previously published animal experiments. We discovered that coadministration with 0.25 mL normal saline solution injected intraperitoneally is associated with a lower mortality rate than calcitriol given alone. The estimated relative risk of mortality was 0.0789 (95% confidence interval, 0.0051−1.22; z = 1.82; P = 0.070) when saline is administered with calcitriol compared to calcitriol alone. There was a reduction in serum calcium levels in mice that received saline (11.4 ± 0.15 mg/dL) compared to mice that did not receive saline (12.42 ± 1.61 mg/dL). Hydration with saline seems to reduce mortality and toxicity in mice receiving calcitriol. Given the decrease in mortality rates, intraperitoneal injections of saline should be considered in studies involving mice receiving injections of calcitriol.