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Journal of Environmental Pathology, Toxicology and Oncology
IF: 1.241 5-Year IF: 1.349 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v27.i3.40
pages 197-207

13-cis-Retinoic Acid Induces Apoptosis by Modulating Caspase-3, bcl-2, and p53 Gene Expression and Regulates the Activation of Transcription Factors in B16F-10 Melanoma Cells

Chandrasekharan Guruvayoorappan
Department of Immunology, Amala Cancer Research Centre, Amala Nagar, Thrissur−680555, Kerala State, India; School of Biotechnology and Health Sciences, Karunya University, Karunya Nagar, Tamil Nadu, India
Chaluvally Raghavan Pradeep
Department of Immunology, Amala Cancer Research Centre, Amala Nagar, Thrissur−680555, Kerala State, India
Girija Kuttan
Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala State, India

ABSTRACT

Cancer prevention using natural products has become an integral part of cancer control. In this study we investigated the effect of 13-cis-retinoic acid on the induction of apoptosis as well as its regulatory effect on the activation of transcription factors in B16F-10 melanoma cells. Treatment of B16F-10 cells with 13-cis-retinoic acid showed the presence of apoptotic bodies and induced DNA fragmentation. 13-cis-retinoic acid treatment also showed an inhibitory effect on bcl-2 expression and upregulated p53 and caspase-3 gene expression in B16F-10 melanoma cells. The study also reveals that 13-cis-retinoic acid treatment could alter the production and expression of proinflammatory cytokines and could inhibit the activation and nuclear translocation of p65, p50, and c-Rel subunits of nuclear factor-nB, and other transcription factors such as c-fos, activated transcription factor-2, and cyclic adenosine monophosphate response element-binding protein in B16F-10 melanoma cells. These results suggest that 13-cis-retinoic acid effectively induces apoptosis in B16F-10 melanoma cells and this compound has the potential as either a therapeutic or chemotherapeutic agent against melanoma.


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