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Journal of Environmental Pathology, Toxicology and Oncology
IF: 1.15 5-Year IF: 1.4 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v25.i4.20
pages 625-632

Electroporation Enhances Radiation and Doxorubicin-Induced Toxicity in Solid Tumor In Vivo

Pratip Shil
Biophysics Laboratory, Department of Physics, University of Pune, Pune 411007 India
Amit Kumar
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India
Pandit B. Vidyasagar
Biophysics Laboratory, Department of Physics, University of Pune, Pune 411007 India
Kaushala Prasad Mishra
Department of Life Sciences, University of Mumbai, Mumbai, India; Nehru Gram Bharati University, Allahabad, UP, India; Foundation for Education and Research, India and BM International Research Centre, Mumbai, India

ABSTRACT

Treatment of cancer patients is subject to limitations in radiotherapy and chemotherapy. This necessitates development of new protocols, and the present work reports on the effects of a combination of local electroporation with ionizing radiation and/or anticancer drug doxorubicin hydrochloride (DOX) on subcutaneous solid tumor murine fibrosarcoma. Localized treatment of fibrosarcoma tumor, grown in right hind leg of Swiss mice, has been carried out using DOX (0.6 mg/kg body weight), radiation (Co 60 γ-rays, dose rate 0.37 Gy/min) and electroporation (1 kV/cm, 200 μs, 8 pulses per burst, 10 bursts) individually or in combinations. Measurements of the tumor growth kinetics after treatment with combinations have revealed significant growth delay. The treatment groups, (i) radiation and electroporation, (ii) DOX and electroporation, and (iii) radiation, DOX and electroporation, have yielded tumor growth delays (TGDs) of 1.22, 1.5, and 1.73 days, respectively, compared to control with the tumor volumes being 53%, 57%, and 49% that of control on the final day of observation. These results suggest that the antitumor effects of a moderate dose of γ radiation and low concentration of DOX can be significantly enhanced by combination with electroporation.