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ISSN Print: 0731-8898
ISSN Online: 2162-6537
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Curcumin, a Potential Inhibitor of MMP-2 in Human Laryngeal Squamous Carcinoma Cells HEp2
ABSTRACT
Curcumin (diferuloylmethane) has been widely studied for its tumor inhibiting and anticarcino-genic properties. In the present communication, we studied the effect of curcumin on matrix-metalloproteinase-2 (MMP-2), integrin receptors, and focal adhesion kinase (FAK) in human laryngeal cancer cells, HEp2. Methods: HEp2 cells were treated with curcumin (5 μM) for 30 days and then grown without curcumin for 28 days. Effect of curcumin on MMP-2 expression and activity and on membrane type matrixmetalloproteinase-1 (MT1-MMP), FAK, and integrin receptors was studied by zymography, Western blot, ELISA, RT-PCR, and cell adhesion assay. Results: Treatment of HEp2 cells with curcumin downregulated MMP-2 expression and activity and expression of integrin receptors, FAK, and MT1-MMP to almost background levels. MMP-2 (but not MMP-9) mRNA expression was abolished on curcumin treatment, indicating specific inhibition of MMP-2. Invasive potential of HEp2 cells was also significantly reduced. After drug withdrawal, expression of MMP-2, integrin receptors, MT1-MMP, and FAK returned to control levels. However, MMP-2 activity in serum free medium remained low. Conclusions: Downregulation of integrin receptors and low levels of FAK may hinder integrin-mediated signal transduction, preventing upregulation of MMP-2 activity. Reduction of MMP-2 activity and inhibition of HEp2 cell invasion by curcumin strongly indicate the potential of curcumin as an inhibitor of tumor cell invasion and metastasis.
-
Sahebkar Amirhossein, Why it is necessary to translate curcumin into clinical practice for the prevention and treatment of metabolic syndrome?, BioFactors, 39, 2, 2013. Crossref
-
Kumar Dileep, Kumar Manish, Saravanan Chinnadurai, Singh Sushil Kumar, Curcumin: a potential candidate for matrix metalloproteinase inhibitors, Expert Opinion on Therapeutic Targets, 16, 10, 2012. Crossref
-
Herbal approaches to pathological states, in Principles and Practice of Phytotherapy, 2013. Crossref
-
Goel Ajay, Kunnumakkara Ajaikumar B., Aggarwal Bharat B., Curcumin as “Curecumin”: From kitchen to clinic, Biochemical Pharmacology, 75, 4, 2008. Crossref
-
Martins Rodrigo Molina, Pereira Simone Vieira, Siqueira Silvia, Salomão Wellington Fioravante, Freitas Luis Alexandre Pedro, Curcuminoid content and antioxidant activity in spray dried microparticles containing turmeric extract, Food Research International, 50, 2, 2013. Crossref
-
Das Tanya, Sa Gaurisankar, Saha Baisakhi, Das Kaushik, Multifocal signal modulation therapy of cancer: ancient weapon, modern targets, Molecular and Cellular Biochemistry, 336, 1-2, 2010. Crossref
-
Ilieva Y., Kaloyanov K., Yosifov D., Robev B., Zhelezova I., Genova M., Mihova A., Balatzenko G., Zaharieva M. M., Berger M. R., Konstantinov S. M., Antineoplastic potential of curcumin (cooperative study in Bulgaria and Germany), Phytochemistry Reviews, 13, 2, 2014. Crossref
-
Sahu R P, Batra S, Srivastava S K, Activation of ATM/Chk1 by curcumin causes cell cycle arrest and apoptosis in human pancreatic cancer cells, British Journal of Cancer, 100, 9, 2009. Crossref
-
Gupta Subash C., Kim Ji Hye, Prasad Sahdeo, Aggarwal Bharat B., Regulation of survival, proliferation, invasion, angiogenesis, and metastasis of tumor cells through modulation of inflammatory pathways by nutraceuticals, Cancer and Metastasis Reviews, 29, 3, 2010. Crossref
-
Weng Chia-Jui, Yen Gow-Chin, Chemopreventive effects of dietary phytochemicals against cancer invasion and metastasis: Phenolic acids, monophenol, polyphenol, and their derivatives, Cancer Treatment Reviews, 38, 1, 2012. Crossref
-
Huang Wu-Yang, Cai Yi-Zhong, Zhang Yanbo, Natural Phenolic Compounds From Medicinal Herbs and Dietary Plants: Potential Use for Cancer Prevention, Nutrition and Cancer, 62, 1, 2009. Crossref
-
Amin A.R.M. Ruhul, Karpowicz Phillip A., Carey Thomas E., Arbiser Jack, Nahta Rita, Chen Zhuo G., Dong Jin-Tang, Kucuk Omer, Khan Gazala N., Huang Gloria S., Mi Shijun, Lee Ho-Young, Reichrath Joerg, Honoki Kanya, Georgakilas Alexandros G., Amedei Amedeo, Amin Amr, Helferich Bill, Boosani Chandra S., Ciriolo Maria Rosa, Chen Sophie, Mohammed Sulma I., Azmi Asfar S., Keith W. Nicol, Bhakta Dipita, Halicka Dorota, Niccolai Elena, Fujii Hiromasa, Aquilano Katia, Ashraf S. Salman, Nowsheen Somaira, Yang Xujuan, Bilsland Alan, Shin Dong M., Evasion of anti-growth signaling: A key step in tumorigenesis and potential target for treatment and prophylaxis by natural compounds, Seminars in Cancer Biology, 35, 2015. Crossref
-
Pavan Aline, Silva Gabriel, Jornada Daniela, Chiba Diego, Fernandes Guilherme, Man Chin Chung, dos Santos Jean, Unraveling the Anticancer Effect of Curcumin and Resveratrol, Nutrients, 8, 11, 2016. Crossref
-
Van Hung Pham, Vo Thi Ngoc Duyen, Structure, physicochemical characteristics, and functional properties of starches isolated from yellow (Curcuma longa ) and black (Curcuma caesia ) turmeric rhizomes, Starch - Stärke, 69, 5-6, 2017. Crossref
-
Shanmugam Muthu, Rane Grishma, Kanchi Madhu, Arfuso Frank, Chinnathambi Arunachalam, Zayed M., Alharbi Sulaiman, Tan Benny, Kumar Alan, Sethi Gautam, The Multifaceted Role of Curcumin in Cancer Prevention and Treatment, Molecules, 20, 2, 2015. Crossref
-
Javeri Indu, Chand Naresh, Curcumin, in Nutraceuticals, 2016. Crossref
-
CHEN QIAN, GAO QING, CHEN KUNLUN, WANG YIDONG, CHEN LIJUAN, LI XU, Curcumin suppresses migration and invasion of human endometrial carcinoma cells, Oncology Letters, 10, 3, 2015. Crossref
-
Fernandes Mirela Garcia, Cervi Camila Bitencourt, Aparecida de Carvalho Rosemary, Lapa-Guimarães Judite, Evaluation of Turmeric Extract as an Antioxidant for Frozen Streaked Prochilod (Prochilodus lineatus) Fillets, Journal of Aquatic Food Product Technology, 26, 9, 2017. Crossref
-
Aggarwal Bharat B., Van Kuiken Michelle E., Iyer Laxmi H., Harikumar Kuzhuvelil B., Sung Bokyung, Molecular Targets of Nutraceuticals Derived from Dietary Spices: Potential Role in Suppression of Inflammation and Tumorigenesis, Experimental Biology and Medicine, 234, 8, 2009. Crossref
-
Tan Cheng, Zhang Li, Cheng Xian, Lin Xiu-Feng, Lu Rong-Rong, Bao Jian-Dong, Yu Hui-Xin, Curcumin inhibits hypoxia-induced migration in K1 papillary thyroid cancer cells, Experimental Biology and Medicine, 240, 7, 2015. Crossref
-
Schmitt Florian, Subramaniam Dharmalingam, Anant Shrikant, Padhye Subhash, Begemann Gerrit, Schobert Rainer, Biersack Bernhard, Halogenated Bis(methoxybenzylidene)-4-piperidone Curcuminoids with Improved Anticancer Activity, ChemMedChem, 13, 11, 2018. Crossref
-
Silva Gabriel, Teixeira Lima Felipe, Seba Viviane, Mendes Lourenço Ana, Lucas Thaise, de Andrade Bianca, Torrezan Guilherme, Polaquini Carlos, Garcia Marcelo, Couto Lucélio, Bestetti Reinaldo, de Castro França Suzelei, Fachin Ana, Regasini Luis, Marins Mozart, Curcumin Analog CH-5 Suppresses the Proliferation, Migration, and Invasion of the Human Gastric Cancer Cell Line HGC-27, Molecules, 23, 2, 2018. Crossref
-
Bachmeier Beatrice, Killian Peter, Melchart Dieter, The Role of Curcumin in Prevention and Management of Metastatic Disease, International Journal of Molecular Sciences, 19, 6, 2018. Crossref
-
Calaf Gloria M., Roy Debasish, Metastatic genes targeted by an antioxidant in an established radiation- and estrogen-breast cancer model, International Journal of Oncology, 51, 5, 2017. Crossref
-
Wan Mohd Tajuddin Wan Nur Baitty, Lajis Nordin H., Abas Faridah, Othman Iekhsan, Naidu Rakesh, Mechanistic Understanding of Curcumin’s Therapeutic Effects in Lung Cancer, Nutrients, 11, 12, 2019. Crossref
-
Zhang Ruiwen, Nag Subhasree, Nutraceuticals in Preventive Oncology: Chemical Biology and Translational Pharmaceutical Science, in Pharma-Nutrition, 12, 2014. Crossref
-
Sharma Prachi, Manchanda Ritu, Goswami Rajesh, Chawla Sanjeev, Biodiversity and Therapeutic Potential of Medicinal Plants, in Environmental Concerns and Sustainable Development, 2020. Crossref
-
Tailor Dhanir, Singh Rana P., Dietary and Non-dietary Phytochemicals in Cancer Control, in Nutrition, Diet and Cancer, 2012. Crossref
-
Sukati Suriyan, Khobjai Warachate, Total Phenolic Content and DPPH Free Radical Scavenging Activity of Young Turmeric Grown in Southern Thailand, Applied Mechanics and Materials, 886, 2019. Crossref
-
Golonko A., Lewandowska H., Świsłocka R., Jasińska U.T., Priebe W., Lewandowski W., Curcumin as tyrosine kinase inhibitor in cancer treatment, European Journal of Medicinal Chemistry, 181, 2019. Crossref
-
Kumaravel Mohankumar, Sankar Pajaniradje, Latha Periyasamy, Benson Chellakan S, Rukkumani Rajagopalan, Antiproliferative Effects of an Analog of Curcumin in Hep-2 cells: A Comparative Study with Curcumin, Natural Product Communications, 8, 2, 2013. Crossref
-
Desam Nagarjuna Reddy, Al-Rajab Abdul Jabbar, Herbal biomolecules: anticancer agents, in Herbal Biomolecules in Healthcare Applications, 2022. Crossref
-
Mohankumar Kumaravel, Francis Arul Prakash, Pajaniradje Sankar, Rajagopalan Rukkumani, Synthetic curcumin analog: inhibiting the invasion, angiogenesis, and metastasis in human laryngeal carcinoma cells via NF-kB pathway, Molecular Biology Reports, 48, 8, 2021. Crossref
-
Wroński Paweł, Wroński Stanisław, Kurant Marcin, Malinowski Bartosz, Wiciński Michał, Curcumin May Prevent Basement Membrane Disassembly by Matrix Metalloproteinases and Progression of the Bladder Cancer, Nutrients, 14, 1, 2021. Crossref
-
Davoodvandi Amirhossein, Farshadi Marjan, Zare Noushid, Akhlagh Seyed Amirreza, Alipour Nosrani Esmail, Mahjoubin-Tehran Maryam, Kangari Parisa, Sharafi Seyedeh Maryam, Khan Haroon, Aschner Michael, Baniebrahimi Ghazaleh, Mirzaei Hamed, Antimetastatic Effects of Curcumin in Oral and Gastrointestinal Cancers, Frontiers in Pharmacology, 12, 2021. Crossref
-
Sharma Garima, Nim Shalu, Alle Madhusudhan, Husen Azamal, Kim Jin-Chul, Nanoparticle-Mediated Delivery of Flavonoids for Cancer Therapy: Prevention and Treatment, in Smart Nanomaterials in Biomedical Applications, 2021. Crossref
-
Arip Masita, Yap Vi Lien, Rajagopal Mogana, Selvaraja Malarvili, Dharmendra K, Chinnapan Sasikala, Evidence-Based Management of Uterine Fibroids With Botanical Drugs-A Review, Frontiers in Pharmacology, 13, 2022. Crossref
-
Mahmoudi Ali, Atkin Stephen L., Jamialahmadi Tannaz, Banach Maciej, Sahebkar Amirhossein, Effect of Curcumin on Attenuation of Liver Cirrhosis via Genes/Proteins and Pathways: A System Pharmacology Study, Nutrients, 14, 20, 2022. Crossref