Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
Journal of Environmental Pathology, Toxicology and Oncology
IF: 1.625 5-Year IF: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v21.i2.50
9 pages

Oxidant-Sensitive Transcription Factor and Cyclooxygenase-2 by Helicobacter pylori Stimulation in Human Gastric Cancer Cells

Hyeyoung Kim
Department of Pharmacology and Institute ofGastroen-terology, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea
Joo Weon Lim
Department of Pharmacology and Institute ofGastroen-terology, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea
Jeong Yeon Seo
Department of Pharmacology and Institute ofGastroen-terology, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea
Kyung Hwan Kim
Department of Pharmacology and Institute ofGastroen-terology, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea

ABSTRACT

Helicobacter pylori (H. pylori) infection might activate nuclear factor-kB (NF-kB), an oxidant-sensitive transcription regulator of inducible expression of inflammatory genes such as cyclooxygenase-2 (COX-2). We studied the role of NF-kB on expression of COX-2 in H. pylori-stimulated gastric cancer cell lines by using antioxidants, glutathione (GSH), and N-acetylcysteine (NAC) as well as an NF-kB inhibitor, pyrrolidine dithiocarbamate (PDTC). Gastric adenocarcinoma cell lines derived from Caucasian (AGS) cells and Korean (SNU-484) cells were used to study the role of NF-kB on COX-2 expression by H. pylori. They were treated with GSH, NAC, or PDTC in the presence of H. pylori. mRNA expression and protein level for COX-2 were determined by Northern blot and RT-PCR analysis as well as Western blot analysis. NF-kB activation was examined by electrophoretic mobility shift assay. As a result, H. pylori induced a time-dependent expression of mRNA and protein for COX-2 via activation of NF-kB, which was inhibited by GSH, NAC, and PDTC in the cells. In conclusion, oxidant-sensitive transcription factor NF-kB may play a novel role in expression of COX-2 by H. pylori stimulation in gastric cancer cells.


Articles with similar content:

In Vitro Evidence of the Role of COX-2 in Attenuating Gastric Inflammation and Promoting Gastric Carcinogenesis
Journal of Environmental Pathology, Toxicology and Oncology, Vol.21, 2002, issue 2
Seonghyang Sohn, Hyuk-Jae Kwon, Young-Joon Surh, Ki-Baik Hahm, Young-Bae Kim, Ki-Myung Lee, Hee-Jae Joo, Seung-Won Cho, Jeong-Sang Lee, Ho-Yeong Lim, Won-Seok Kim
Effects of Yakuchinone A and Yakuchinone В on the Phorbol Ester-Induced Expression of COX-2 and iNOS and Activation of NF-kB in Mouse Skin
Journal of Environmental Pathology, Toxicology and Oncology, Vol.21, 2002, issue 2
Young-Joon Surh, Ok Hee Kim, Jee-Young Kang, Hoil Kang, Kyung-Soo Chun
Protective Effect of Solanum muricatum on Tumor Metastasis by Regulating Inflammatory Mediators and Nuclear Factor-Kappa B Subunits
Journal of Environmental Pathology, Toxicology and Oncology, Vol.34, 2015, issue 3
Chandrasekaran Guruvayoorappan, Kittappa Shathish, Kunnathur Murugesan Sakthivel
Oleanolic acid induces Apoptosis by modulating p53, Bax, Bcl-2 and caspase-3 gene expression and regulates the activation of transcription factors and cytokine profile in B16F
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 1
P. Pratheeshkumar, Girija Kuttan
Features and Functions of gd T Lymphocytes: Focus on Chemokines and Their Receptors
Critical Reviews™ in Immunology, Vol.23, 2003, issue 5-6
Dieter Kabelitz, Daniela Wesch