Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
Journal of Environmental Pathology, Toxicology and Oncology
IF: 1.15 5-Year IF: 1.4 SJR: 0.519 SNIP: 0.613 CiteScore™: 1.61

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.2018025137
pages 53-62

Evaluation of Antiarthritic Activity of Butanol Fraction of Punica granatum Linn. Rind Extract Against Freund’s Complete Adjuvant-Induced Arthritis in Rats

Rupesh Kumar Gautam
Department of Pharmacology, MM School of Pharmacy, MM University Sadopur-Ambala, India
Sanjay Sharma
NMIMS, School of Pharmacy and Technology Management, Shirpur, Maharashtra, India
Komal Sharma
Department of Pharmacology, Bhupal Nobles' Institute of Pharmaceutical Sciences, Udaipur, India
Gaurav Gupta
School of Pharmacy, Suresh Gyan Vihar University, Jaipur, India

ABSTRACT

Available conventional drugs produce several side effects; thus, a potent herbal drug is urgently required for the management of rheumatoid arthritis. The present investigation was performed to evaluate the antiarthritic activity of butanol fraction of Punica granatum Linn. rind methanolic extract (PGBF) against Freund's complete adjuvant (FCA)–induced arthritis in rats.
An acute toxicity study of butanol fraction was conducted, accompanied by a study of its antiarthritic activity. Chromatography (thin layer chromatography and high-performance thin layer chromatography) analyses were also conducted. Phytochemical screening of the fraction was performed to confirm the presence of phytoconstituents. For antiarthritic activity, the active butanol fraction was administered at doses of 50 and 75 mg/kg body weight. The antiarthritic activity was evaluated by using biophysical paramaters (arthritic score, body weight, paw volume and joint diameter) and hematological parameters [red blood cell (RBC) count, white blood cell (WBC) count, hemoglobin (Hb) concentration and erythrocyte sedimentation rate (ESR)]. Dexamethasone (5 mg/kg) was selected as the standard.
Phytochemical screening of butanol fraction showed the presence of steroids, tannins, flavonoids, irioid glycosides, and phenolic compounds. Acute toxicity studies suggested that butanolic fraction was safe up to a dose of 500 mg/kg. The data regarding biophysical and hematological parameters clearly indicated that the butanol fraction at a 75 mg/kg dose showed a more significant effect (**P<0.01 and ***P<0.001) than the 50 mg/kg dose.
The antiarthritic potential of a butanol fraction of Punica granatum Linn. rind extract may be due to the presence of active phytoconstituents such as flavonoids, irioid glycosides, and phenolic compounds. Future studies will provide a new approach in relation to the antiarthritic activity of Punica granatum Linn., and the isolation of its active compound may eventually lead to the development of a new category of the antiarthritic agent.


Articles with similar content:

Antiarthritic Activity of Diallyl Disulfide against Freund’s Adjuvant–Induced Arthritic Rat Model
Journal of Environmental Pathology, Toxicology and Oncology, Vol.37, 2018, issue 4
Xiaochen Jin, Yuqing Chen, Xiangqi Tan, Rong Xue
Structural Characterization of Polysaccharides of a Productive Strain of the Culinary-Medicinal King Oyster Mushroom, Pleurotus eryngii (Agaricomycetes), from Italy
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 8
Paola Saporita, Giuseppe Bonaventura, Maria Letizia Gargano, Roberta Calvo, Marina Zacchigna, Giuseppe Venturella, Francesca Cateni, Giuseppe Procida, Celeste Caruso Bavisotto
In Vivo Iron-Chelating Activity and Phenolic Profiles of the Angel's Wings Mushroom, Pleurotus porrigens (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 9
Masoumeh Khalili, Mohammad Ali Ebrahimzadeh, Mehrnoush Kosaryan
Antioxidant, Anti-inflammatory, and Antitumor Activities of Culinary-Medicinal Mushroom Pleurotus pufmonanus (Fr.) Quel. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.4, 2002, issue 4
Thekkuttuparambil Ananthanarayanan Ajith, Kainoor K. Janardhanan, Nayana Jose
Antioxidant Properties of Oak Bracket Mushroom, Pseudoinonotus dryadeus (Higher Basidiomycetes): A Mycochemical Study
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 7
Marina Zacchigna, Tiziano Altieri, Angelo Cichelli, Francesca Cateni, Giuseppe Procida