Published 4 issues per year
ISSN Print: 0731-8898
ISSN Online: 2162-6537
Indexed in
Antibacterial Medicinal Plants Equiseti Herba and Ononidis Radix Modulate Micronucleus Formation in Human Lymphocytes In Vitro
ABSTRACT
We assessed the in vitro cytogenetic effects of extracts of the commonly used medicinal plants Equiseti herba, Ononidis radix, and Uva ursi on irradiated human blood lymphocytes. We examined the acquired micronucleus formation in unirradiated and irradiated samples of cultured blood lymphocytes using the cytochalasin block micronucleus test (CBMN). Centromere-positive micronuclei were identified by fluorescence in situ hybridization using a DNA probe labeled with a-satellite digogsigenin. Equiseti herba had weak clastogenic properties, increasing the yield of micronuclei in unirradiated samples and reducing the level of radiation-induced micronuclei in a concentration-dependent manner. In the control, unirradiated samples, 36.8% of micronuclei were centromerepositive (MNC+), while in the irradiated ones the percentage of MNC+ ranged from 10.8-15.3%, indicating a clastogenic mechanism for the micronuclei formation. Ononidis radix was a strong clastogen and radiosensitizer, rapidly increasing the yield of micronuclei in unirradiated samples up to 5-fold and potentiating the yield of radiation-induced micronuclei up to 1.7-fold. In cultures treated with Ononidis radix, the percentage of MNC+ micronuclei ranged from 18.8 to 23.8%, indicating that micronuclei originated by a clastogenic mechanism. Uva ursi did not affect the yield of micronuclei either in unirradiated or in irradiated samples. The micronucleus formation assay is a reliable screen for plant extracts and purified compounds, for the identification of compounds that might either inhibit clastogenesis or potentiate radiotherapy for malignancy.
-
Kuntić Vesna S., Stanković Miroslava B., Vujić Zorica B., Brborić Jasmina S., Uskoković-Marković Snežana M., Radioprotectors - the Evergreen Topic, Chemistry & Biodiversity, 10, 10, 2013. Crossref
-
Štajner Dubravka, Popović Boris M., Čanadanović-Brunet Jasna, Anačkov Goran, ExploringEquisetum arvenseL.,Equisetum ramosissimumL. andEquisetum telmateiaL. as sources of natural antioxidants, Phytotherapy Research, 23, 4, 2009. Crossref
-
Milovanović Vesna, Radulović Niko, Todorović Zoran, Stanković Miroslava, Stojanović Gordana, Antioxidant, Antimicrobial and Genotoxicity Screening of Hydro-alcoholic Extracts of Five Serbian Equisetum Species, Plant Foods for Human Nutrition, 62, 3, 2007. Crossref
-
Materia Medica, in Veterinary Herbal Medicine, 2007. Crossref
-
Harutyunyan Karina, Balayan Karine, Tadevosyan Gohar, Hayrapetyan Manush, Musayelyan Ruzanna, Grigoryan Ruzanna, Khondkaryan Lusine, Sarkisyan Natalya, Babayan Nelly, Genotoxic potential of selected medicinal plant extracts in human whole blood cultures, Journal of Herbmed Pharmacology, 8, 2, 2019. Crossref
-
Joksić Gordana, Tričković Jelena Filipović, Joksić Ivana, Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases, Current Pharmaceutical Design, 25, 18, 2019. Crossref
-
Gallo Francesca R., Multari Giuseppina, Federici Elena, Palazzino Giovanna, Giambenedetti Massimo, Petitto Valentina, Poli Ferruccio, Nicoletti Marcello, Chemical fingerprinting of Equisetum arvense L. using HPTLC densitometry and HPLC, Natural Product Research, 25, 13, 2011. Crossref
-
Jurica Karlo, Karačonji Irena Brčić, Benković Vesna, Kopjar Nevenka, In vitro assessment of the cytotoxic, DNA damaging, and cytogenetic effects of hydroquinone in human peripheral blood lymphocytes, Archives of Industrial Hygiene and Toxicology, 68, 4, 2017. Crossref
-
Dormousoglou Margarita, Efthimiou Ioanna, Antonopoulou Maria, Fetzer Damian L., Hamerski Fabiane, Corazza Marcos L., Papadaki Maria, Santzouk Samir, Dailianis Stefanos, Vlastos Dimitris, Investigation of the Genotoxic, Antigenotoxic and Antioxidant Profile of Different Extracts from Equisetum arvense L., Antioxidants, 11, 7, 2022. Crossref