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Journal of Environmental Pathology, Toxicology and Oncology

Impact factor: 1.246

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvPathToxOncol.v22.i1.60
10 pages

Studies on Correlation of Antimutagenic and Antiproliferative Activities of Juglans regia L.

Kamaljit Kaur
Department of Botanical Sciences, Guru Nanak Dev University, Amritsar, Punjab, India
Husheem Michael
Department of Anatomy, ! e Institute of Biomedicine, University of Turku, Turku, Finland
Saroj Arora
Department of Botanical Sciences, Guru Nanak Dev University, Amritsar 143 005, Punjab, India
Pirkko L. Harkonen
Department of Anatomy, ! e Institute of Biomedicine, University of Turku, Turku, Finland
Subodh Kumar
Department of Chemistry, Guru Nanak Dev University, Amritsar, Punjab, India

ABSTRACT

We investigated the effect of water and acetone extract of Juglans regia L. to evaluate its antimutagenic and antiproliferative activities. The antimutagenic study using TA98 and TA100 tester strains of Salmonella revealed the water and acetone extracts to be more effective than the benzene and chloroform extracts in inhibiting the revertants induced by 2–aminoflourene (2AF) in TA100 tester strains. The most effective extracts in the Ames assay were further evaluated using the Lucifer luciferase assay and in time course studies for antiproliferative activities using the Hoechst staining to observe apoptotic cell deaths. The acetone extract showed a correlation of antimutagenic activities in the Ames assay with its antiproliferative effect in different cell lines, while the water extract exerted its effect distinctly in each cell line. Further studies are still needed to evaluate the cytotoxicity in experiments carried out in vivo.