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Journal of Environmental Pathology, Toxicology and Oncology

Impact factor: 1.107

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvPathToxOncol.v23.i2.70
8 pages

Evaluation of Radioprotective Action of Compounds Using Saccharomyces cerevisiae

Purva Nemavarkar
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India
Bishnavath K. Chourasia
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India
Karpagam Pasupathy
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India

ABSTRACT

The yeast Saccharomyces cerevisiae serves as a model eukaryotic system to screen radioprotectors that can be used primarily in radiotherapy as well as in occupational workers in nuclear and allied industries. A number of antioxidants are suggested to be radioprotectors by virtue of their ability to quench reactive oxygen species, but their radioprotective action has not been investigated so far. In this study, a number of antioxidants were tested for their efficacy in radioprotection using yeast cells. Sulfhydryl compounds (disulfiram at 100 and 200 μM) and reduced glutathione (10 and 100 mM), purified compounds of plant origin, such as curcumin (1 mM, 10 mM, and 100 mM), quercetin (100 and 500 μM), rutin (100 and 200 μM), ellagic acid (100,200, and 500 μM) and gallic acid (100 and 500 μM)—were studied. The results revealed that all compounds, except gallic acid, specifically protected normal yeast cells from γ-radiation damage. Using rad 52 mutants, which lack recombinational DNA repair pathway, it has been found that protection was solely brought about by reducing DNA damage rather than by interfering with DNA repair. Results with DNA repair polymerase further substantiated this contention. We conclude that simple eukaryotic yeast cells can serve as a test system not only for rapid screening of radiomodifiers but also to study their mode of action.