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Journal of Environmental Pathology, Toxicology and Oncology

Impact factor: 0.877

ISSN Print: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvPathToxOncol.v23.i2.60
6 pages

Radioprotective Effect of Podophyllotoxin in Saccharomyces cerevisiae

Madhu Bala
Division of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Delhi, 110054, India
Harish Chandra Goel
Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Delhi, India

ABSTRACT

Recent reports showed that whole extract of Podophyllum hexandrum was radioprotective in mice. 1 Podophyllotoxin is one of the major constituents of the whole extract of Podophyllum. In this study we report on the radioprotective action of podophyllotoxin in Saccharomyces cerevisiae yeast. Proliferating yeast cells pretreated with podophyllotoxin (2.5-5.0 μg/mL) for ≥3 hours showed a higher surviving fraction after 60Co-γ-irradiation (200-600 Gy) than did the irradiated cells not pretreated with podophyllotoxin. The maximum increase (2.0 times) in surviving fraction was observed in cells treated with 2.5 μg/mL podophyllotoxin, 5 hours before 60Co-γ-irradiation (400 Gy). Podophyllotoxin was not mutagenic or recombinogenic at radioprotective doses (2.5 μg/mL). A post-irradiation decrease in revertants and gene convertants was observed in cells treated with podophyllotoxin (2.5 μg/mL podophyllotoxin, -5 hours, 400 Gy). This study indicates that podophyllotoxin is radioprotective in yeast, and its radioprotective effects in higher eukaryotes would be worth investigating.