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Nanoscience and Technology: An International Journal
ESCI SJR: 0.219 SNIP: 0.484 CiteScore™: 0.37

ISSN Print: 2572-4258
ISSN Online: 2572-4266

Nanoscience and Technology: An International Journal

Formerly Known as Nanomechanics Science and Technology: An International Journal

DOI: 10.1615/NanomechanicsSciTechnolIntJ.v4.i1.40
pages 59-66

COLLECTION, ISOLATION, IDENTIFICATION, AND BIOSYNTHESIS OF SILVER NANOPARTICLES USING MICROALGA CHLORELLA PYRENOIDOSA

Sanniyasi Elumalai
Department of Plant Biology & Biotechnology, Presidency College, Chennai-05, INDIA
B. Infant Santhose
Department of Plant Biology & Biotechnology, Presidency College, Chennai-05, INDIA
R. Devika
Department of Plant Biology & Biotechnology, Presidency College, Chennai-05, INDIA
S. Revathy
Department of Plant Biology & Biotechnology, Presidency College, Chennai-05, INDIA

ABSTRACT

The samples were collected from rock ponds in Kovalam, isolated with the aid of plating techniques, and identified at the Krishnamurthy Institute of Algology, Chennai. Algal cells were grown in a Bolds Basal medium and cultured for 14 days for 16:8 h at 24°C. In 14 days the algal cells were pelleted by centrifugation. The bioreduction of Ag+ ions in solution was monitored by means of the UV-visible spectroscopy from zero reading, and doubly distilled water was used as a blank. The samples were withdrawn at various time intervals and the absorbance was measured at 450 nm. The silver nanoparticles synthesized using extracts of algal samples was confirmed by color changes and was characterized by a UV-visible spectrophotometer; the UV-visible spectra showed a broad peak located at 450 nm for silver nanoparticles. The TEM analysis shows small spherical 5−10-nm-sized particles. The nanoparticles were also characterized by the SEM, EDAX, XRD, and FTIR analysis.


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