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Forum on Immunopathological Diseases and Therapeutics
SJR: 0.164 SNIP: 0.041 CiteScore™: 0.18

ISSN Print: 2151-8017
ISSN Online: 2151-8025

Archives: Volume 1, 2010 to Volume 7, 2016

Forum on Immunopathological Diseases and Therapeutics

DOI: 10.1615/ForumImmunDisTher.2011004397
pages 227-236

Gene Expression Profile in Murine 3T3 Fibroblasts Photosensitized by a Tetracationic Porphyrin

Monica Camerin
Department of Biology, University of Padova, Italy
Marina Soncin
Department of Biology, University of Padova, Padova, Italy
Ester Piccinni
Department of Biology, University of Padova, Italy
Paola Irato
Department of Biology, University of Padova, Italy
Francesco Boldrin
Department of Biology, University of Padova, Italy
Laura Guidolin
Department of Biology, University of Padova, Padova, Italy
Giulio Jori
Department of Biology, University of Padova, Via Ugo Bassi 58B, 35121 Padova, Italy
Gianfranco Santovito
Department of Biology, University of Padova, Italy

ABSTRACT

The interest regarding the study of cell death in the context of photodynamic therapy (PDT) is quite vigorous. The phenomena of apoptosis, autophagy, and necrosis have been observed thereby, and the specific results depend on the treatment parameters and cell line. To identify key genes expressed in response to photosensitization after short-term exposure to porphyrins and visible light, a study of the transcriptome of murine 3T3 fibroblast cell cultures was performed using the microarray approach. We studied the effect induced by a tetracationic mesosubstituted porphyrin (C12) in this cell line, employing different incubation times and photosensitizer concentrations. A limited mortality (approximately 5%) was obtained by irradiating cells (1 min, 10 mW/cm2) incubated with 0.1 μM C12 for 1 h, hence these samples are suitable for studying initial gene expression. The analysis of microarray data produced the identification of 521 differentially expressed genes in treated cells with respect to controls, 57 of which were overexpressed. Among these, the 25% are involved in cell proliferation and differentiation processes. Notably, one of the overexpressed genes, the tumour suppressor candidate 1 (tusc 1), was expressed 3-fold more in photosensitized fibroblasts.