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Journal of Long-Term Effects of Medical Implants

ISSN Print: 1050-6934
ISSN Online: 1940-4379

Journal of Long-Term Effects of Medical Implants

DOI: 10.1615/JLongTermEffMedImplants.v13.i4.80
8 pages

Comparative Study of the Covered Area of Leeds–Keio (LK) Artificial Ligament and Radio Frequency Generated Glow Discharge Treated Leeds–Keio (Bio-LK) Ligament with Synovial Cells

Satoshi Tsukazaki
Department of Orthopaedic Surgery, National Defense Medical Collage, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan
Toshiyuki Kikuchi
Department of Orthopaedic Surgery, National Defense Medical Collage, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan
Kyosuke Fujikawa
Knee Disease Centre, Ohtsuki Hospital for Surgery, Tokyo; Professor of Orthopaedic Surgery. Department of Orthopredic Surgery National Defense Medical College, Saitama, Japan
Tatsuo Kobayashi
Department of Orthopaedic Surgery, National Defense Medical Collage, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan
Bahaa B. Seedhom
Rheumatology and Rehabilitation Research Unit, University of Leeds, 36 Clarendon Road, Leeds LS 2 9NZ, NK

ABSTRACT

The Leeds-Keio artificial ligament (LK), which was developed not only as a ligament substitute but also as a scaffold for tissue induction in knee ligament reconstruction, has been in clinical use since 1982 in Europe and Japan. Recently, we have developed radio frequency generated glow discharge (RFGD)-treated LK ligament (Bio-LK) to expedite the process of tissue induction and its maturation. In this study of cell adhesion to the scaffold, we report the difference in the covered area with synovial cells when using scaffolds made from treated and untreated materials. Plasma clot methods were used in this study. The covered area on LK and Bio-LK by cells was stained by 0.1% toluidine blue and analyzed using NIH image. The covered area of Bio-LK was about three times higher than that of LK (untreated) at 3 weeks. In scanning electron microscopy, more cells were observed on fibers of Bio-LK, and these filled the space among the fibers more extensively. The spreading of covered area means that cell attachment, cell proliferation, and cell migration on the fibers are likely to be improved. Our experimental study indicates that Bio-LK will possibly speed up the process of induction of autogenous tissue from synovium.