Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
Journal of Long-Term Effects of Medical Implants

ISSN Print: 1050-6934
ISSN Online: 1940-4379

Journal of Long-Term Effects of Medical Implants

DOI: 10.1615/JLongTermEffMedImplants.v21.i1.60
pages 71-77

Effect of Bioactive Ceramic and Recombinant Human Transforming Growth Factor-Beta (rhTGF-beta) on Regeneration of Parietal Bone Defects in Rat

Fariborz Nasseri
Periodontics Department, Dental School, Shahid Beheshti University of Medical Sciences, Daneshju Blvd., Tehran, Iran
Gholam Ali Gholami
Periodontics Department, Dental School, Shahid Beheshti University of Medical Sciences, Daneshju Blvd., Tehran, Iran
Mahdi Kadkhodazadeh
Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

ABSTRACT

Aims: The purpose of this study was to evaluate the regenerative response of parietal bone defects in rats to bioactive ceramic and TGF-beta 1. Materials and Methods: Sixty mature adult rats with initial weights of 300 g to 324 g were used. The diameter of each defect was 3 mm. The animals were divided into four study groups consisting of 15 rats per group. Groups 1 and 3 (control groups) received 30 μg bioceramic per surgical defect site. Groups 2 and 4 (experimental groups) received 30 μg bioceramic combined with 10 μL of reconstituted TGF-beta 1 (200 ng). Animals of Groups 1 and 3 were euthanized at 18 days; Group 2 and 4 animals were terminated at 49 days after surgery. Each of the outcomes was analyzed via two-way analysis of variance model (ANOVA). Results: Histologic reactions to the implants were similar with no significant difference between bioceramic and bioceramic + TGF-beta 1 specimens. In the 18-day specimens, the percentage of ceramic was marginally higher than the 49-day specimens. No significant difference was noted in the percentage of bone in 18-day and 49-day specimens. Conclusions: Based on the findings of this study, it was concluded that TGF-beta 1 promoted no significant increase in new bone when compared to bioactive ceramic alone.