Published 4 issues per year
ISSN Print: 1050-6934
ISSN Online: 1940-4379
Indexed in
Design of a Tribocorrosion Bioreactor for the Analysis of Immune Cell Response to in Situ Generated Wear Products
ABSTRACT
Adverse local tissue reactions to wear debris and corrosion products have lead to a sharp decline in the use of metal-on-metal (MOM) total hip athroplasties (THAs) clinically. Today, approximately 1 million patients are still carrying such a device. To gain a better understanding of the effect of wear and corrosion products on cells within the joint environment, it is important to generate conditions in vitro that resemble the in vivo system as closely as possible. In this paper, we present a novel tribocorrosion bioreactor that enables the simultaneous conduction of tribocorrosion and cell-culture experiments. In this setup, macrophage cell cultures are located in direct proximity to a tribological interface mimicking the sliding conditions of THA and are exposed to wear and corrosion products as they are generated. These products may include meta-stable species and metallo-organic complexes that have not been considered in earlier studies. The combination of standard tribological, electrochemical, and biological techniques is associated with several challenges that are described here in detail.
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Villanueva J., Trino L., Thomas J., Bijukumar D., Royhman D., Stack M. M., Mathew M. T., Corrosion, Tribology, and Tribocorrosion Research in Biomedical Implants: Progressive Trend in the Published Literature, Journal of Bio- and Tribo-Corrosion, 3, 1, 2017. Crossref
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Peroglio Marianna, Gaspar Diana, Zeugolis Dimitrios I., Alini Mauro, Relevance of bioreactors and whole tissue cultures for the translation of new therapies to humans, Journal of Orthopaedic Research, 2017. Crossref
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Rani Bijukumar Divya, Segu Abhijith, Mou YongChao, Ghodsi Reza, Shokufhar Tolou, Barba Mark, Li Xue-Jun, Thoppil Mathew Mathew, Differential toxicity of processed and non-processed states of CoCrMo degradation products generated from a hip simulator on neural cells, Nanotoxicology, 12, 9, 2018. Crossref
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Figueiredo-Pina C.G., Rodrigues I., Sequeira J., Guedes M., Carneiro C., Does the presence of a S. Salivarius biofilm influence the tooth-zirconia pair triboactivity? An in-vitro study, Wear, 430-431, 2019. Crossref
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Bijukumar Divya Rani, Salunkhe Shruti, Morris Dalton, Segu Abhijith, Hall Deborah J., Pourzal Robin, Mathew Mathew T., In Vitro Evidence for Cell‐Accelerated Corrosion Within Modular Junctions of Total Hip Replacements, Journal of Orthopaedic Research, 38, 2, 2020. Crossref
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Radice S., Westrick J., Ebinger K., Mathew T.M., Wimmer M.A., In-vitro studies on cells and tissues in tribocorrosion processes: A systematic scoping review, Biotribology, 24, 2020. Crossref
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Radice S., Holcomb T., Pourzal R., Hallab N.J., Laurent M.P., Wimmer M.A., Investigation of CoCrMo material loss in a novel bio-tribometer designed to study direct cell reaction to wear and corrosion products, Biotribology, 18, 2019. Crossref
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Radice Simona, Wimmer Markus A., Biotribometer for Assessment of Cell and Tissue Toxicity of Orthopedic Metal Implant Debris, in Biomedical Engineering Technologies, 2394, 2022. Crossref