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Journal of Long-Term Effects of Medical Implants
CiteScore™: 0.89 SNIP: 0.491 SJR: 0.332

ISSN Print: 1050-6934
ISSN Online: 1940-4379

Journal of Long-Term Effects of Medical Implants

DOI: 10.1615/JLongTermEffMedImplants.2014010305
pages 65-76

Design of a Tribocorrosion Bioreactor for the Analysis of Immune Cell Response to in Situ Generated Wear Products

Robin Pourzal
Rush University Medical Center, Chicago, IL
R. Cichon
University of Duisburg-Essen, Duisburg, Germany
M. T. Mathew
Rush University Medical Center, Chicago, IL
C. A. Pacione
Rush University Medical Center, Chicago, IL
A. Fischer
Rush University Medical Center, Chicago, IL ; University of Duisburg-Essen, Duisburg, Germany
Nadim James Hallab
Orthopedic Analysis, LLC, Department of Immunology, Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL 60612
Markus Wimmer
Rush University Med Ctr

ABSTRACT

Adverse local tissue reactions to wear debris and corrosion products have lead to a sharp decline in the use of metal-on-metal (MOM) total hip athroplasties (THAs) clinically. Today, approximately 1 million patients are still carrying such a device. To gain a better understanding of the effect of wear and corrosion products on cells within the joint environment, it is important to generate conditions in vitro that resemble the in vivo system as closely as possible. In this paper, we present a novel tribocorrosion bioreactor that enables the simultaneous conduction of tribocorrosion and cell-culture experiments. In this setup, macrophage cell cultures are located in direct proximity to a tribological interface mimicking the sliding conditions of THA and are exposed to wear and corrosion products as they are generated. These products may include meta-stable species and metallo-organic complexes that have not been considered in earlier studies. The combination of standard tribological, electrochemical, and biological techniques is associated with several challenges that are described here in detail.