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Journal of Long-Term Effects of Medical Implants

ISSN Print: 1050-6934
ISSN Online: 1940-4379

Journal of Long-Term Effects of Medical Implants

DOI: 10.1615/JLongTermEffMedImplants.v13.i1.30
10 pages

Glove Powder’s Carrying Capacity for Latex Protein: Analysis Using the ASTM ELISA Test

Don Beezhold
Senior Scientist. Guthrie Research Institute One Guthrie Square Sayre PA 18840
K. Horton
Guthrie Research Institute, Sayre, Pennsylvania
V. Hickey
Guthrie Research Institute, Sayre, Pennsylvania
J. Daddona
Guthrie Research Institute, Sayre, Pennsylvania
D. Kostyal
Guthrie Research Institute, Sayre, Pennsylvania

ABSTRACT

Glove donning powders carry latex proteins and disperse them into the workplace environment. We have used the ASTM D6499 ELISA to quantify the amount of latex antigen bound to and carried by glove powders. We could differentiate between a small amount of protein actually bound to the powders and a larger amount carried by the powder. Enhanced binding of a major allergen, Hev b 5, to the starch powders was demonstrated by Western blot. The D6499 ELISA is able to measure total latex antigen, soluble and powder bound, simultaneously without the need to centrifuge the samples.