Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
Critical Reviews™ in Immunology
IF: 1.404 5-Year IF: 3.347 SJR: 0.706 SNIP: 0.55 CiteScore™: 2.19

ISSN Print: 1040-8401
ISSN Online: 2162-6472

Volume 40, 2020 Volume 39, 2019 Volume 38, 2018 Volume 37, 2017 Volume 36, 2016 Volume 35, 2015 Volume 34, 2014 Volume 33, 2013 Volume 32, 2012 Volume 31, 2011 Volume 30, 2010 Volume 29, 2009 Volume 28, 2008 Volume 27, 2007 Volume 26, 2006 Volume 25, 2005 Volume 24, 2004 Volume 23, 2003 Volume 22, 2002 Volume 21, 2001 Volume 20, 2000 Volume 19, 1999 Volume 18, 1998 Volume 17, 1997 Volume 16, 1996 Volume 15, 1995 Volume 14, 1994

Critical Reviews™ in Immunology

DOI: 10.1615/CritRevImmunol.v18.i5.30
pages 449-484

DNA Vaccines

Wayne C. Lai
Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
Michael Bennett
Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235


DNA vaccination against infectious diseases has created a new field of applied molecular immunology. cDNAs for 'protective' protein epitopes can be inserted into vectors containing strong mammalian promoters for high expression. Here we discuss the mechanisms of DNA vaccination and the successful and sometimes unsuccessful applications of DNA vaccination to protect animals against many different viral, bacterial mycoplasmal, protozoal, and worm infections or infestations. DNA immunization has been used to prevent or inhibit tumor development and to inhibit IgE responses by diverting the immune response from Th2 to Th1 helper cell dominance. Advantages and disadvantages of a variety of routes of administration and methods of immunization discussed include the use of the 'gene gun', the delivery of genes by aerosols, and deliberate induction of injury to muscles prior to injection of DNA to enhance gene expression. Vaccination performed using DNA without knowing beforehand the protective epitopes, using 'expression library immunization', is discussed. While this field is bound to expand rapidly for future clinical applications, we try to point out potential pitfalls as well as advantages of this relatively new technology.

Articles with similar content:

Mucosal Immunization: A Review of Strategies and Challenges
Critical Reviews™ in Therapeutic Drug Carrier Systems, Vol.31, 2014, issue 4
Mohan N. Rathi, Hinal Patel, Chetan Yewale, Ambikanandan Misra
Recognition of Melanoma-Derived Antigens by CTL: Possible Mechanisms Involved in Down-Regulating Anti-Tumor T-Cell Reactivity
Critical Reviews™ in Immunology, Vol.18, 1998, issue 1-2
Flavio Arienti, Alessandro Borsatti, Paola Squarcina, Chiara Castelli, Filiberto Belli, Ettore Appella, Francesca Rini, Douglas J. Loftus, Francesco M. Marincola, Giorgio Parmiani, Licia Rivoltini, Carsten Geisler
Biopolymers as Transdermal Drug Delivery Systems in Dermatology Therapy
Critical Reviews™ in Therapeutic Drug Carrier Systems, Vol.27, 2010, issue 2
M.A. Navya, K. H. Basavaraj, Siddaramaiah, George Johnsy, R. Rashmi
T Cell Receptors for Gene Transfer in Adoptive T Cell Therapy
Critical Reviews™ in Immunology, Vol.39, 2019, issue 2
David M. Kranz, Preeti Sharma
Role of Galectin-3 in the Initial Control of Leishmania Infection
Critical Reviews™ in Immunology, Vol.34, 2014, issue 2
Guillaume St-Pierre, Pampa Bhaumik, Isabelle Pelletier, Sachiko Sato