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Hydrobiological Journal

ISSN Print: 0018-8166
ISSN Online: 1943-5991

Hydrobiological Journal

DOI: 10.1615/HydrobJ.v50.i1.80
pages 100-106

Peculiarities of Diagnostics of the Functional State of the Unicellular Algae in the Liquid Media by Luminescent Method

O. V. Vakulenko
Taras Shevchenko National University Kyiv, Ukraine
O. O. Grigorieva
Taras Shevchenko Kiev National University, Kiev, Ukraine
M. A. Berezovskaya
Taras Shevchenko Kiev National University, Kiev, Ukraine
A. I. Datsenko
Taras Shevchenko National University Kyiv, Ukraine

ABSTRACT

Peculiarities of application of the luminescent methods for determination of the functional state of the unicellular algae in the liquid media are considered in brief. Use of traditional method of the fluorescence induction in this case is complicated; however diagnostics can be carried out by static intensity and spectra of the chlorophyll α photoluminescence at the room and low temperature. Luminescence parameters are noted which can be used for assessment of the cells functional state in culture. Length of excitation wave should be taken into account. The method is proposed enabling to mitigate effect of fluorescence induction on measurement results.