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Critical Reviews™ in Biomedical Engineering

ISSN Print: 0278-940X
ISSN Online: 1943-619X

Critical Reviews™ in Biomedical Engineering

DOI: 10.1615/CritRevBiomedEng.v36.i5-6.50
pages 441-471

Hybrid Superporous Scaffolds: An Application for Cornea Tissue Engineering

Arpita Kadakia
Department of Bioengineering, University of Illinois, Chicago, IL, USA
Vandana Keskar
Department of Biopharmacuetical Sciences, University of Illinois, Chicago, IL, USA
Igor A. Titushkin
Bioengineering Department, University of Illinois, Chicago, USA
Ali Djalilian
Department of Ophthalmology and Visual Science, University of Illinois, Chicago, IL, USA
Richard A. Gemeinhart
Department of Bioengineering, and Department of Biopharmacuetical Sciences, University of Illinois, Chicago, IL, USA
Michael Cho
Department of Bioengineering, University of Illinois, Chicago, IL, USA

ABSTRACT

Engineering a cell-based keratoprosthesis often requires a struggle between two essential parameters: natural 3-D biological adhesion and mechanical strength. A novel hybrid scaffold of natural and synthetic materials was engineered to achieve both cell adhesion and implantable strength. This scaffold was characterized in terms of cell adhesion, cell migration, swelling, and strength. While the study was focused on engineering a biointegrable prosthetic skirt, a clear central core with an appropriate refractive index and light transmission was also incorporated into the design for potential functionality. The hybrid scaffold was tested in rat corneas. This uniquely designed scaffold was well tolerated and encouraged host cell migration into the implant. The hybrid superporous design also enhanced cell adhesion and retention in a superporous scaffold without altering the bulk mechanical properties of the hydrogel.