Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
Critical Reviews™ in Biomedical Engineering
SJR: 0.207 SNIP: 0.376 CiteScore™: 0.79

ISSN Print: 0278-940X
ISSN Online: 1943-619X

Critical Reviews™ in Biomedical Engineering

DOI: 10.1615/CritRevBiomedEng.v31.i56.20
66 pages

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Nianzhen Li
Department of Bioengineering, University of Washington, Seattle, Washington
Anna Tourovskaia
Department of Bioengineering, University of Washington, Seattle, Washington
Albert Folch
Department of Bioengineering, University of Washington, Seattle, Washington


The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology−consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium−has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Microfabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

Articles with similar content:

Transcriptional Analysis of Tumor-Specific T-Cell Responses in Cancer Patients
Critical Reviews™ in Immunology, Vol.22, 2002, issue 5-6
Ena Wang, Jos Even, Dirk Nagorsen, Francesco M. Marincola
Tumor Cell Recognition by Lymphocytes: Is the MHC Always Essential?
Critical Reviews™ in Immunology, Vol.18, 1998, issue 1-2
Beverly Z. Packard , Akira Komoriya
Polymeric Gene Carriers
Critical Reviews™ in Eukaryotic Gene Expression, Vol.15, 2005, issue 4
Han Chang Kang, You Han Bae, Minhyung Lee
Adipose-Derived Stromal Vascular Fraction Cells: Update on Clinical Utility and Efficacy
Critical Reviews™ in Eukaryotic Gene Expression, Vol.25, 2015, issue 2
Ki-Chul Hwang, Hyun Min Sun, Seongho Han, Sung-Whan Kim
Efflux Time Comparison for Tanks of Different Geometries
International Journal of Fluid Mechanics Research, Vol.38, 2011, issue 1
C. Bhaskara Sarma, V. S. R. K. Prasad, S. Kishore, Ch. V. Subbarao, P. V. Gopal Singh