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Plasma Medicine

Published 4 issues per year

ISSN Print: 1947-5764

ISSN Online: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Viability and Cell Biology for HeLa and Vero Cells after Exposure to Low-Temperature Air Dielectric Barrier Discharge Plasma

Volume 7, Issue 2, 2017, pp. 159-173
DOI: 10.1615/PlasmaMed.2017019487
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ABSTRACT

Cancer study is among the hottest topics in plasma medicine. Differential killing of cancer cells and controllable selectivity may be achieved using exposure to plasma generated by various atmospheric pressure discharges. In this article, we discuss the possibility of generating low-temperature air plasma directly inside standard 24- and 96-well cell-culture plates and its subsequent use to achieve selective cytotoxicity. After exposure, cell viability was assessed and molecular biology tests carried out to understand the effect of plasma on cell biology. HeLa (neoplastic cells) and Vero (normal cells) cultures were exposed to air dielectric barrier discharge plasma. We performed viability assessment using the 3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide assay at 8 and 24 hr after treatment and found a reduction in cell viability, with a variable amplitude of effect. The cytotoxicity of plasma on HeLa cells at 8 hr was 59.92% and reached 68.58% by 24 hr. The cytotoxic effect on Vero cells was limited to 37.75% at 8 hr and only 6.33% at 24 hr. General cell biology analyses were assessed using flow cytometry and gene expression tracking.

CITED BY
  1. Kawasaki Toshiyuki, Nishida Keisuke, Uchida Giichiro, Mitsugi Fumiaki, Takenaka Kosuke, Koga Kazunori, Setsuhara Yuichi, Shiratani Masaharu, Effects of surrounding gas on plasma-induced downward liquid flow, Japanese Journal of Applied Physics, 59, SH, 2020. Crossref

  2. GAUTAM Saurav, MORRA Gabriele, Pre-breakdown to stable phase and origin of multiple current pulses in argon dielectric barrier discharge, Plasma Science and Technology, 23, 12, 2021. Crossref

  3. Raud Sirli, Raud Jüri, Jõgi Indrek, Piller Carl-Thomas, Plank Toomas, Talviste Rasmus, Teesalu Tambet, Vasar Eero, The Production of Plasma Activated Water in Controlled Ambient Gases and its Impact on Cancer Cell Viability, Plasma Chemistry and Plasma Processing, 41, 5, 2021. Crossref

  4. Mihai Cosmin-Teodor, Mihaila Ilarion, Pasare Maria Antoanela, Pintilie Robert Mihai, Ciorpac Mitica, Topala Ionut, Cold Atmospheric Plasma-Activated Media Improve Paclitaxel Efficacy on Breast Cancer Cells in a Combined Treatment Model, Current Issues in Molecular Biology, 44, 5, 2022. Crossref

  5. Caba Bogdan, Gardikiotis Ioannis, Topala Ionut, Mihaila Ilarion, Mihai Cosmin Teodor, Luca Catalina, Pasca Sorin, Caba Ioana Cezara, Dimitriu Gabriel, Huzum Bogdan, Serban Ionela Lacramioara, Cold Atmospheric Plasma, Platelet-Rich Plasma, and Nitric Oxide Synthesis Inhibitor: Effects Investigation on an Experimental Model on Rats, Applied Sciences, 12, 2, 2022. Crossref

  6. Trimukhe Ajinkya M., Pandiyaraj K. N., Patekar Mukesh, Miller Vandana, Deshmukh Rajendra R., Perspectives and Advances of Nonthermal Plasma Technology in Cancers, IEEE Transactions on Plasma Science, 50, 8, 2022. Crossref

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