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Plasma Medicine

Published 4 issues per year

ISSN Print: 1947-5764

ISSN Online: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Bactericidal Efficacy of Dielectric Barrier Discharge Plasma on Methicillin-Resistant Staphylococcus aureus and Escherichia coli in Planktonic Phase and Colonies In vitro

Volume 5, Issue 1, 2015, pp. 1-16
DOI: 10.1615/PlasmaMed.v5.i1.10
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ABSTRACT

Increasing emergence of antibiotic resistance in bacteria during the past few decades has become a major public health concern and has reduced the efficacy of conventional therapies. The objective of the current study is to examine the bactericidal efficacy of atmospheric pressure nonthermal dielectric barrier discharge (DBD) plasma on methicillin-resistant Staphylococcus aureus (MRSA) as a common musculoskeletal pathogen and Escherichia coli (E. coli) as another cause of bone, joint, and soft tissue infections in planktonic phase and in colonies. In planktonic phase, results demonstrated complete decontamination of E. coli and MRSA cells on agar plates with a bacterial surface density of 1.27 × 105 colony-forming units (CFU)/cm2 following 30 seconds and 60 seconds of plasma treatment, respectively. Reduction rates of 7 log10 steps in the number of viable CFUs in E. coli colonies was observed following 5 minutes of plasma treatment. The colony growth was halted, and no more growth in colony size was observed during a 24-hour monitoring period. For MRSA colonies, results demonstrated 2 log10 to 7 log10 steps reduction in the number of viable CFUs in each colony after 5 minutes of plasma treatment. Results indicated that plasma-treated colonies will have prolonged lag time during generation of the growth curves. Finally, diffusion of nitric oxide into the agarose gel was confirmed as a reactive agent that was effective in the decontamination process.

CITED BY
  1. Karki Surya B, Gupta Tripti Thapa, Yildirim-Ayan Eda, Eisenmann Kathryn M, Ayan Halim, Investigation of non-thermal plasma effects on lung cancer cells within 3D collagen matrices, Journal of Physics D: Applied Physics, 50, 31, 2017. Crossref

  2. Karki Surya B., Yildirim-Ayan Eda, Eisenmann Kathryn M., Ayan Halim, Miniature Dielectric Barrier Discharge Nonthermal Plasma Induces Apoptosis in Lung Cancer Cells and Inhibits Cell Migration, BioMed Research International, 2017, 2017. Crossref

  3. Gupta Tripti Thapa, Ayan Halim, Application of Non-Thermal Plasma on Biofilm: A Review, Applied Sciences, 9, 17, 2019. Crossref

  4. Karki Surya B., Gupta Tripti Thapa, Yildirim-Ayan Eda, Eisenmann Kathryn M., Ayan Halim, Miniature Non-thermal Plasma Induced Cell Cycle Arrest and Apoptosis in Lung Carcinoma Cells, Plasma Chemistry and Plasma Processing, 40, 1, 2020. Crossref

  5. Patinglag Laila, Melling Louise M., Whitehead Kathryn A., Sawtell David, Iles Alex, Shaw Kirsty J., Non-thermal plasma-based inactivation of bacteria in water using a microfluidic reactor, Water Research, 201, 2021. Crossref

  6. Ding Tian, Xu Enbo, Liao Xinyu, Inactivation of Bacteria by Cold Plasma, in Applications of Cold Plasma in Food Safety, 2022. Crossref

  7. Ciarolla Alexa A., Lapin Norman, Williams Dustin, Chopra Rajiv, Greenberg David E., Physical Approaches to Prevent and Treat Bacterial Biofilm, Antibiotics, 12, 1, 2022. Crossref

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