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Plasma Medicine

Published 4 issues per year

ISSN Print: 1947-5764

ISSN Online: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Amine-Rich Cell-Culture Surfaces for Research in Orthopedic Medicine

Volume 1, Issue 2, 2011, pp. 115-133
DOI: 10.1615/PlasmaMed.2011003130
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ABSTRACT

We report results of ongoing research relating to cell immobilization and cell culture on bioactive organic coatings deposited using three different techniques−two plasma assisted, the third based on vacuum-ultraviolet photo-polymerization. We start by briefly comparing those three methods and by describing some of the key characteristics of the resulting coatings; all are designed to be rich in primary amines, a functional group known to be highly bioactive. Next, we focus on two cell types of importance to long-term objectives in our orthopedic research laboratory: (i) The first, nonadherent human U937 monocytes, is a cell line that has been widely used as a model of the mammalian cellular response to various inflammatory stimuli, and in understanding the clinical relevance of elevated cobalt and chromium levels in patients with metal-on-metal total hip arthroplasty and hip resurfacing arthroplasty. (ii) The second cell type, adherent human mesenchymal stem cells (MSCs), derived from patients suffering from osteoarthritis (OA), is important in biological repair of cartilage and of the degenerate intervertebral disc (IVD) from the patients' autologous cells. Much progress has been achieved in both cases, as illustrated with results based to a considerable extent on real-time reverse-transcription (RT) polymerase chain reaction (PCR), a key methodology used in this type of research.

CITED BY
  1. Wertheimer Michael R., Plasma Processing and Polymers: A Personal Perspective, Plasma Chemistry and Plasma Processing, 34, 3, 2014. Crossref

  2. Vandenbossche Marianne, Dorst Johanna, Amberg Martin, Schütz Urs, Rupper Patrick, Heuberger Manfred, Hegemann Dirk, Functionality and chemical stability of plasma polymer films exhibiting a vertical cross-linking gradient in their subsurface, Polymer Degradation and Stability, 156, 2018. Crossref

  3. Harumningtyas Anjar Anggraini, Suprapto , Suprihatin Hari, Aziz Ihwanul, Andriyanti Wiwien, Sujitno Tjipto, Purwadi Agus, Hamaguchi Satoshi, Development of a DC-plasma source for surface functionalization by amino groups, PROCEEDINGS OF INTERNATIONAL CONFERENCE ON NUCLEAR SCIENCE, TECHNOLOGY, AND APPLICATION 2020 (ICONSTA 2020), 2381, 2021. Crossref

  4. Kodama Joe, Harumningtyas Anjar Anggraini, Ito Tomoko, Michlíček Miroslav, Sugimoto Satoshi, Kita Hidekazu, Chijimatsu Ryota, Ukon Yuichiro, Kushioka Junichi, Okada Rintaro, Kamatani Takashi, Hashimoto Kunihiko, Tateiwa Daisuke, Tsukazaki Hiroyuki, Nakagawa Shinichi, Takenaka Shota, Makino Takahiro, Sakai Yusuke, Nečas David, Zajíčková Lenka, Hamaguchi Satoshi, Kaito Takashi, Amine modification of calcium phosphate by low-pressure plasma for bone regeneration, Scientific Reports, 11, 1, 2021. Crossref

  5. Dorst J., Vandenbossche M., Amberg M., Bernard L., Rupper P., Weltmann K.-D., Fricke K., Hegemann D., Improving the Stability of Amino-Containing Plasma Polymer Films in Aqueous Environments, Langmuir, 33, 40, 2017. Crossref

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