Library Subscription: Guest
Plasma Medicine

Published 4 issues per year

ISSN Print: 1947-5764

ISSN Online: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

Indexed in

Repeated Cold Atmospheric Plasma Application to Intact Skin Does Not Cause Sensitization in a Standardized Murine Model

Volume 7, Issue 4, 2017, pp. 383-393
DOI: 10.1615/PlasmaMed.2017019167
Get accessDownload

ABSTRACT

Cold atmospheric plasma (CAP) is a promising option for the treatment of chronic wounds and superficial cancer. Recent studies show that CAP has excellent microbicidal and antibiofilm activity, stimulates angiogenesis and cell proliferation, and induces apoptosis in cancer cells. The kINPen MED transportable plasma jet is a certified medical device that needs to be tested for irritant and allergenic side effects. We used a murine local lymph node assay (according to the Organisation for Economic Co-operation and Development protocol) to evaluate the kINPen MED's potential to trigger skin sensitization. Our results showed that 25 s of CAP exposure did not induce skin sensitization compared with the vehicle control (acetone/olive oil mixture). The CAP-treated group had a stimulation index (SI) of 0.76, which was significantly lower than the > 1.8 threshold (SI = 0.46 for the vehicle control group). Pure argon gas flow also did not induce sensitization. Moreover, we did not observe any serious effects on proliferation activity in local lymph nodes (assessed via visual analysis, adenosine triphosphate content, and histology). This study confirmed that the use of the kINPen MED device did not cause any harmful sensitization or irritation in a murine model.

CITED BY
  1. Nguyen Ly, Lu Peng, Boehm Daniela, Bourke Paula, Gilmore Brendan F., Hickok Noreen J., Freeman Theresa A., Cold atmospheric plasma is a viable solution for treating orthopedic infection: a review, Biological Chemistry, 400, 1, 2018. Crossref

  2. Bekeschus Sander, Seebauer Christian, Wende Kristian, Schmidt Anke, Physical plasma and leukocytes – immune or reactive?, Biological Chemistry, 400, 1, 2018. Crossref

  3. Boehm Daniela, Bourke Paula, Safety implications of plasma-induced effects in living cells – a review of in vitro and in vivo findings, Biological Chemistry, 400, 1, 2018. Crossref

  4. Bender Claudia, Kramer Axel, Stope Matthias B., Application in Veterinary Medicine, in Comprehensive Clinical Plasma Medicine, 2018. Crossref

  5. Jablonowski Lukasz, Kocher Thomas, Schindler Axel, Müller Karolina, Dombrowski Frank, von Woedtke Thomas, Arnold Thomas, Lehmann Antje, Rupf Stefan, Evert Matthias, Evert Katja, Eickholz Peter, Side effects by oral application of atmospheric pressure plasma on the mucosa in mice, PLOS ONE, 14, 4, 2019. Crossref

  6. Rödder Katrin, Moritz Juliane, Miller Vandana, Weltmann Klaus-Dieter, Metelmann Hans-Robert, Gandhirajan Rajesh, Bekeschus Sander, Activation of Murine Immune Cells upon Co-culture with Plasma-treated B16F10 Melanoma Cells, Applied Sciences, 9, 4, 2019. Crossref

  7. Bekeschus Sander, Clemen Ramona, Nießner Felix, Sagwal Sanjeev Kumar, Freund Eric, Schmidt Anke, Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion, Advanced Science, 7, 10, 2020. Crossref

  8. Ng Sing Wei, Tsoukou Evanthia, Chaple Sonal, Boehm Daniela, Bourke Paula, Plasma Treatment of Liquids, in Innovative Food Processing Technologies, 2021. Crossref

  9. Bekeschus Sander, Bauer Georg, Miller Vandana, Immunology in Plasma Cancer Treatment, in Plasma Cancer Therapy, 115, 2020. Crossref

  10. Bekeschus Sander, Kramer Axel, Schmidt Anke, Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine, Molecules, 26, 18, 2021. Crossref

  11. Boekema Bouke, Stoop Matthea, Vlig Marcel, van Liempt Jos, Sobota Ana, Ulrich Magda, Middelkoop Esther, Antibacterial and safety tests of a flexible cold atmospheric plasma device for the stimulation of wound healing, Applied Microbiology and Biotechnology, 105, 5, 2021. Crossref

  12. Schmidt Anke, Bekeschus Sander, How Safe is Plasma Treatment in Clinical Applications?, in Textbook of Good Clinical Practice in Cold Plasma Therapy, 2022. Crossref

  13. Miebach Lea, Poschkamp Broder, van der Linde Julia, Bekeschus Sander, Medical Gas Plasma—A Potent ROS-Generating Technology for Managing Intraoperative Bleeding Complications, Applied Sciences, 12, 8, 2022. Crossref

  14. Peng Sansan, Qi Miao, Zhang Huaiyan, Zhang Jin, Liu Rong, Pang Bolun, Zhang Xinying, Liu Zhijie, Zhang Hao, Liu Dingxin, Xu Dehui, Discharge characteristics of a microsecond pulse power supply driven air plasma jet and its anticancer cell effect, Physics of Plasmas, 29, 1, 2022. Crossref

  15. Trimukhe Ajinkya M., Pandiyaraj K. N., Patekar Mukesh, Miller Vandana, Deshmukh Rajendra R., Perspectives and Advances of Nonthermal Plasma Technology in Cancers, IEEE Transactions on Plasma Science, 50, 8, 2022. Crossref

  16. Matthias B Stope, Non-invasive physical plasma as an innovative physical approach for the oncological therapy of skeletal sarcomas, Journal of Radiology and Oncology, 6, 3, 2022. Crossref

Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections Prices and Subscription Policies Begell House Contact Us Language English 中文 Русский Português German French Spain