Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
Plasma Medicine
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN Print: 1947-5764
ISSN Online: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2014011720
pages 1-10

Comparative Study on the Use of Different Metal Electrodes in Low-Pressure Glow Discharge Plasma Sterilization

Joanna Abigael Daseco
Plasma Physics Laboratory, National Institute of Physics, University of the Philippines, Diliman Quezon City 1101, Philippines
Karel G. Pabelina
Plasma Physics Laboratory, National Institute of Physics, University of the Philippines, Diliman Quezon City 1101, Philippines
Ma. Auxilia T. Siringan
Microbiological Research and Services Laboratory, Natural Science and Research Institute, University of the Philippines, Diliman Quezon City 1101, Philippines
Henry J. Ramos
Plasma Physics Laboratory, National Institute of Physics, University of the Philippines, Diliman Quezon City 1101, Philippines

ABSTRACT

The sterilizing efficacy of low-pressure direct current glow discharge hydrogen peroxide (H2O2) plasma generated by a planar parallel plate plasma source using the plasma enhanced chemical vapor deposition (PECVD) facility was tested. This study compares the effect of using different metals (copper, stainless steel, and aluminum) as electrodes in plasma sterilization of stainless steel dishes. Test samples were exposed to H2O2 plasma under different sets of discharge currents and exposure times. Bacillus subtilis was used as the test organism and microbial analysis was made by means of the standard plate count method of serial dilution and pour plating. Evaluation of microbial death was done using survival curves, percent reduction, and decimal reduction value. Results showed that sterilization using copper electrodes exhibited the highest decimal reduction value (D-value) and percent reduction among the three electrodes. However, statistical analysis using multivariate analysis of variance (MANOVA) at 0.05 significance level assessed that the type of electrode material is not a significant factor in the H2O2 plasma sterilization of Bacillus subtilis cells.


Articles with similar content:

Optimization of Short-Pulsed Dielectric Barrier Discharge for In-Package Disinfection
Plasma Medicine, Vol.8, 2018, issue 2
Gregory Fridman, Alyssa Suarez, Anita Mannsberger, Chong Liu, Alexander A. Fridman, Ngoc Anh Huynh, Vandana Miller, Pietro Ranieri
Surfatron-Produced Atmospheric-Pressure Plasma Jet Applied to Candida Biofilms
Plasma Medicine, Vol.8, 2018, issue 4
Homero S. Maciel, Fernanda R. Figueira, Anelise C.O.C. Doria, J. S. B. Lima, Sonia Khouri, S. Pessoa
Ex Vivo Study Comparing Three Cold Atmospheric Plasma (CAP) Sources for Biofilm Removal on Microstructured Titanium
Plasma Medicine, Vol.9, 2019, issue 1
Ann Cathrin Poehlmann, Saskia Preissner, Olaf Nell, Antje Lehmann, Thomas Arnold, Andreas Schubert, Stefan Rupf
EXPERIMENTAL INVESTIGATIONS ON PRECISION MACHINING OF THERMAL BARRIER COATINGS AND APPLICATION OF THE GREY RELATION APPROACH TO DETERMINE THE OPTIMUM PROCESS PARAMETERS
High Temperature Material Processes: An International Quarterly of High-Technology Plasma Processes, Vol.20, 2016, issue 4
Mohammad S. Alsoufi, Mohammed Yunus
Action of an Argon/Water Vapor Plasma Jet in the Sterilization of Silicone Contaminated with Candida albicans
Plasma Medicine, Vol.7, 2017, issue 3
Jhonatan Steffens Brandao Lima, Homero Santiago Maciel, Fernanda Ramos Figueira, Anelise C.O.C. Doria, Ligia Satiko Simomura, Sonia Khouri, Guilherme Torello Cassiano Redi, Rodrigo Savio Pessoa