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Atomization and Sprays
IF: 1.189 5-Year IF: 1.596 SJR: 0.814 SNIP: 1.18 CiteScore™: 1.6

ISSN Print: 1044-5110
ISSN Online: 1936-2684

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Atomization and Sprays

DOI: 10.1615/AtomizSpr.2017020134
pages 847-858

EXPERIMENTAL INVESTIGATION OF ENDOSCOPIC CELL SPRAY

Malte Bieber
Institute of Heat and Mass Transfer, RWTH Aachen University, 52062 Aachen, Germany
Anja Lena Thiebes
Department of Biohybrid & Medical Textiles (BioTex) at AME-Helmholtz Institute for Biomedical Engineering and ITA-Institut für Textiltechnik, RWTH Aachen University, 52074 Aachen, Germany
Christian Gabriel Cornelissen
Department for Internal Medicine Section for Pneumology, Medical Faculty, RWTH Aachen University Hospital, 52074 Aachen, Germany
Stefan Jockenhoevel
Department of Biohybrid & Medical Textiles (BioTex) at AME-Helmholtz Institute for Biomedical Engineering and ITA-Institut für Textiltechnik, RWTH Aachen University, 52074 Aachen, Germany
Reinhold Kneer
Institute of Heat and Mass Transfer, RWTH Aachen University, Augustinerbach 6, 52062 Aachen, Germany
Manuel Armin Reddemann
Institute of Heat and Mass Transfer, RWTH Aachen University, Augustinerbach 6, 52056 Aachen, Germany

ABSTRACT

Stem cell therapy requires high local concentration of living cells at the area of treatment. An innovative approach aims at high local cell concentrations in hollow organs by application of cells through flexible endoscopy. The endoscope's working channel is equipped with a catheter nozzle for coaxial liquid atomization of cell suspensions. Until now, little information is available regarding the influence of atomization parameters on cell survival and the influence of cells in solution on atomization. Therefore, a parametric study on atomization of cell suspensions is performed in this work. The mutual interaction of cells and atomization is experimentally analyzed, aiming at a correlation between air Reynolds number, droplet sizes, and cell survival rates. The influences of catheter flow, atomization, and droplet wall interaction on cell survival are quantified and thus the extensional flow during ligament formation is identified as main cell destruction mechanism. On the basis of the present endoscopic configuration, integral cell survival rates above 80% are achieved. Thus, it offers the opportunity for homogeneous local distribution of cells in hollow cavities.