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Critical Reviews™ in Eukaryotic Gene Expression

Impact factor: 1.660

ISSN Print: 1045-4403
ISSN Online: 2162-6502

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukaryotGeneExpr.v13.i24.130
16 pages

Identification and Characterization of the Genes Encoding Human and Mouse Osteoactivin

Thomas A. Owen
Department of Theoretical and Applied Science, Ramapo College of New Jersey, Mahwah, New Jersey; and Department of Cardiovascular and Metabolic Diseases , Pfizer Global Research and Development - Groton Laboratories, Groton, CT 06340, USA
Steven L. Smock
Department of Cardiovascular and Metabolic Diseases, Pfizer Global Research and Development - Groton Laboratories, Groton, CT 06340
S. Prakash
Department of Cardiovascular and Metabolic Diseases, Pfizer Global Research and Development - Groton Laboratories, Groton, CT 06340
L. Pinder
Department of Genomic and Proteomic Sciences, Pfizer Global Research and Development - Groton Laboratories, Groton, CT 06340
D. Brees
Department of Pathology, Pfizer Global Research and Development - Groton Laboratories, Groton, CT 06340
D. Krull
Department of Pathology, Pfizer Global Research and Development - Groton Laboratories, Groton, CT 06340
T. A. Castleberry
Department of Cardiovascular and Metabolic Diseases, Pfizer Global Research and Development - Groton Laboratories, Groton, CT 06340
Y. C. Clancy
Department of Cardiovascular and Metabolic Diseases, Pfizer Global Research and Development - Groton Laboratories, Groton, CT 06340
Sandy C. Marks, Jr.
Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655, USA
Fayez F. Safadi
Department of Anatomy and Cell Biology, Department of Orthopaedic Surgery and Sport Medicine, Department of Otolaryngology, Temple University School of Medicine, Philadelphia, PA, USA
Steven N. Popoff
Department of Anatomy and Cell Biology, and Department of Orthopaedic Surgery and Sport Medicine, Temple University School of Medicine, Philadelphia, PA 19140, USA

ABSTRACT

Osteoactivin (OA) is more highly expressed in the bones of osteopetrotic mutant rats (op/op) than in those of their normal littermates and is the homologue of human nmb, a cDNA more highly expressed in melanoma-derived cell lines of low metastatic potential, and of mouse DC-HIL, which has been implicated in endothelial cell adhesion. The human OA gene is found on chromosome 7p15.1 and consists of 11 exons spanning 28.3 kb. Murine OA is encoded by a highly similar gene of 11 exons spanning 20.2 kb on mouse chromosome 6. Human OA uses the same transcriptional initiation site in both bone and kidney as was reported for melanoma cells. OA is expressed in primary human and mouse osteoblast cultures at all stages of differentiation, with increased levels observed concurrently with the expression of osteoblast phenotype markers. OA is also expressed in a wide variety of human and mouse tissues as determined by RT-PCR analysis. Immunohistochemical investigation of OA expression in late mouse embryonic development showed very high, cell-specific expression in the nervous system, basal layer of the skin, germinal cells of hair follicles, and in the forming nephrons of the kidney. Continuing investigation of the cell-specific expression of OA in bone as well as in other tissues will lead to a better understanding of its function in the development of these cell types.