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Critical Reviews™ in Eukaryotic Gene Expression
IF: 1.841 5-Year IF: 1.927 SJR: 0.627 SNIP: 0.516 CiteScore™: 1.96

ISSN Print: 1045-4403
ISSN Online: 2162-6502

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukaryotGeneExpr.2019025189
pages 69-76

Calcium-Sensing Receptor Arbitrates Hypoxia-Induced Proliferation of Pulmonary Artery Smooth Muscle Cells via the G Protein-PLC-IP3 Pathway

Hui Zhang
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People's Republic of China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan 430070, People's Republic of China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Zhenyu Chang
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Khalid Mehmood
University College of Veterinary and Animal Sciences, Islamia University of Bahawalpur-63100, Pakistan
Keli Yang
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People's Republic of China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan 430070, People's Republic of China
Zewen Liu
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People's Republic of China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan 430070, People's Republic of China
Zhengying Duan
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People's Republic of China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan 430070, People's Republic of China
Fangyan Yuan
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People's Republic of China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan 430070, People's Republic of China
Zuowu Jiao
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People's Republic of China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan 430070, People's Republic of China
Wei Liu
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People's Republic of China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan 430070, People's Republic of China
Ting Gao
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People's Republic of China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan 430070, People's Republic of China
Xiaoying Pei
Wuhan General Team of Agricultural Comprehensive Law Enforcement Supervision, Wuhan 430014, People's Republic of China
Muhammad Ijaz
Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore-54600, Pakistan
Muhammad Taslim Ghori
University College of Veterinary and Animal Sciences, Islamia University of Bahawalpur-63100, Pakistan
Yongxiang Tian
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People's Republic of China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan 430070, People's Republic of China
Rui Guo
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, 430064, People's Republic of China; Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan 430070, People's Republic of China

ABSTRACT

Pulmonary arterial hypertension (PAH), also known as broilers ascites syndrome, is characterized by hypoxia, pulmonary artery pressure, and right heart failure. However, less information is available about the molecular mechanisms of PAH. We evaluated the mediation of calcium-sensing receptor by inducing hypoxia for the possible proliferation of pulmonary artery smooth muscle cells via the G protein pathway. For this purpose, we used an in vitro trial of chicken cell culture and confirmed our results by using immunohistochemistry, immunofluorescence staining, quantitative real-time polymerase chain reaction assay, and Western blotting analysis. Our results showed that the mRNA and protein expression levels of calcium-sensing receptor (CaSR) were significantly upregulated in cells when co-incubated with CaCl2. However, the levels of mRNA and protein were obviously decreased when supplemented with blocking agents (NiCl2, 2-APB, and D609). Furthermore, the experimentally induced hypoxia also upregulated the expression of CaSR gene as compared to CaSR gene expression in control cells. Together, these results indicate that hypoxia plays an important role in the expression of CaSR gene in pulmonary artery smooth muscle cells and reveals new targets for the CaSR excited hypothesis to prevent and control PAH in chickens.


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