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Critical Reviews™ in Eukaryotic Gene Expression

Impact factor: 1.660

ISSN Print: 1045-4403
ISSN Online: 2162-6502

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukarGeneExpr.v17.i4.10
pages 259-269

The Phosphoinositide-Phospholipase C (PI-PLC) Pathway in the Mouse Oocyte

Brigitte Lefevre
INSERM U566/CEA/DRR/LGAG/Univ Paris 7, Batiment 05, route du Panorama, F-92260, Fontenay-aux-roses, France
Arlette Pesty
INSERM U566/CEA/DRR/LGAG/Univ Paris 7, Batiment 05, route du Panorama, F-92260, Fontenay-aux-roses, France
Anne-Marie Courtot
INSERM U566/CEA/DRR/LGAG/Univ Paris 7, Batiment 05, route du Panorama, F-92260, Fontenay-aux-roses, France
Catarina Vaz Carreto Martins
INSERM U566/CEA/DRR/LGAG/Univ Paris 7, Batiment 05, route du Panorama, F-92260, Fontenay-aux-roses, France
Ophelie Broca
La Pitié Salpêtrière, UF Biologie de la Reproduction, Paris, France
Anne Denys
INSERM Eri-18, Univ Paris 13, Bobigny, France
Emilie Arnault
INSERM U566/CEA/DRR/LGAG/Univ Paris 7, Batiment 05, route du Panorama, F-92260, Fontenay-aux-roses, France
Catherine Poirot
La Pitié Salpêtrière, UF Biologie de la Reproduction, Paris, France
Nathalie Avazeri
Société Bracer-Biotech, INRA, Jouy-en-Josas, France

ABSTRACT

As highlighted in this review, the phosphoinositide-phospholipase C pathway is strongly implicated in the control of mouse oocyte meiosis. The pathway becomes progressively functional as oocyte growth advances, and it appears to play a role in the G2/M transition when meiosis resumes, at least in the in vitro spontaneous model. Even if the inositol 1,4,5-trisphosphate receptors are present from the beginning, they function and release Ca2+ when the follicular antrum appears. Phospholipase C β1 (PLCβ1) is first exclusively localized to the nucleus and then migrates to the cytoplasm when the oocyte is fully grown. During oocyte maturation PLCβ1 is active in the cytoplasm before it migrates and becomes active in the nucleus just prior to germinal vesicle breakdown. Because a similar circuit is observed for protein kinase C α (PKCα), PKCβ1, PKCβ2, and active mitogen-activated protein kinase, it is tempting to envisage that a feedback loop occurs between these pathways as demonstrated in other cell types. The chronology of these molecular movements into the oocyte reveals the particular and important role of the nucleus phosphoinositide cycle during oocyte meiosis. It appears also that this chronology is crucial and that defects leading to an inappropriate intracellular localization can have dramatic consequences. Such anomalies can prevent the production of competent oocytes and lead to fertility problems.