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International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Print: 2155-014X
ISSN Online: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v8.i1.20
pages 13-23

Functional Activity of Ehrlich Ascites Carcinoma Cells after Treatment with Hybrid Nanocomplexes, Containing Orthovanadates of Rare Earth Elements, Cholesterol and Luminescent Dye

Anatoliy N. Goltsev
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
Nataliya A. Babenko
Institute of Biology, Kharkiv Karazin National University, Kharkiv, Ukraine; Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
Yuliya A. Gaevskaya
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
Olga V. Chelombitko
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
Nikolay A. Bondarovich
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
Tatyana G. Dubrava
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
Maksim V. Ostankov
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
Vladimir K. Klochkov
Institute for Scintillation Materials of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
Nataliya S. Kavok
Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine; Institute for Scintillation Materials of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
Yuriy V. Malyukin
Institute for Scintillation Materials of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

ABSTRACT

Tumor development is the consequence of expanding the population of low differentiated cells with unlimited self-maintenance potential, i.e. cancer stem cells (CSCs). Application of new forms of nanocomposites capable of binding to CSCs and inducing the tumor destruction is a promising direction for treating this pathology. There have been developed the methods of obtaining hybrid nanocomplexes containing rare-earth orthovanadates GdYVO4: Eu3+, cholesterol, and luminescent dye Dil. By immune fluorescence method using monoclonal antibodies to CD44, CD24, CD117 and Sca-1 markers, there has been established the change in the ratio of tumor progenitors of various differentiation levels in a general pool of Ehrlich ascites carcinoma (EAC) after treatment with hybrid nanocomplexes. Essential reduction of the concentration of the most tumorogenic CD44high cells with simultaneous rise in the number of CD117+-cells results in an increase in the index of CD44high / CD117+ ratio. It has been demonstrated that an application of hybrid nanocomplexes suppresses the tumor growth by almost 80%. The value of cooperative interactions of the cells with different phenotype signs in tumor sites has been proved. The index of CD44high / CD117+ ratio can be used as one of diagnostic and prognostic parameters of the development and inactivation rate of tumor process when using different types of anti-tumor therapy.


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