Published 4 issues per year
ISSN Print: 2155-014X
ISSN Online: 2155-0158
Energy-Related Function of the Mitochondria from Rat Cardiomyocytes in Artificial Hypobiosis
ABSTRACT
We examined energy-related mitochondrial activity of the rat cardiomyocytes under artificial carbon dioxide-induced hypobiosis, which led to physiological changes in the body (a decrease in body temperature, heart rate reduction, etc). The mitochondrial respiratory and phosphorylating activities of cardiomyocytes are reduced when using two oxidation substrates, namely succinate and malate, characterizing the rate of oxygen consumption by the mitochondria. The partial uncoupling of oxidation / phosphorylation processes by malate was found, unlike succinate. The activity of NADH-KoQ-oxidoreductase (complex I of the respiratory chain) is inhibited, whereas the activities of succinate dehydrogenase and cytochrome oxidase remain unchanged. Predominant use of succinate under the artificial hypobiosis seems to promote mitochondrial energy-related activity, as evidenced by the ATP synthesizing activity of the mitochondria. Modifications of the structural and functional state of cardiomyocyte inner mitochondrial membrane are adaptive under the artificial hypobiosis.