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International Journal of Physiology and Pathophysiology

Published 4 issues per year

ISSN Print: 2155-014X

ISSN Online: 2155-0158

SJR: 0.116

Effect of Anti-NaPi2b Monoclonal Antibody on Phosphate Transport in Renal Cancer Cell Line SK-RC-18

Volume 2, Issue 3, 2011, pp. 279-289
DOI: 10.1615/IntJPhysPathophys.v2.i3.80
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ABSTRACT

The sodium-dependent phosphate transporter NaPi2b (SLC34A2, NaPi-IIb, NPT2b) is involved in regulation of inorganic phosphate metabolism. Its expression was detected in a set of normal and malignant tissues predominantly on mRNA level. Here we describe NaPi2b protein expression in tubules of human kidney by using immunohistochemical analysis with anti-NaPi2b mAb. Patch-clamp technique was used to study effect of anti-NaPi2b mAb on sodium-dependent phosphate current in renal cancer cells (SK-RC-18 cell line) expressing high level of NaPi2b protein. We demonstrate that incubation of SK-RC-18 cells with anti-NaPi2b mAb decreases phosphate current in 70% cells while unspecific antibody A33 does not produce any effect. Thus, anti-NaPi2b mAb can serves as a potential specific inhibitor of NaPi2b mediated phosphate transport. NaPi2b and anti-NaPi2b mAb have a great potential for diagnosis and treatment of different pathologies related to aberrant expression and function of NaPi2b phosphate transporter.

CITED BY
  1. Bulatova Leisan, Savenkova Daria, Nurgalieva Alsina, Reshetnikova Daria, Timonina Arina, Skripova Vera, Bogdanov Mikhail, Kiyamova Ramziya, Toward a Topology-Based Therapeutic Design of Membrane Proteins: Validation of NaPi2b Topology in Live Ovarian Cancer Cells, Frontiers in Molecular Biosciences, 9, 2022. Crossref

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