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International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Print: 2155-014X
ISSN Online: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v7.i4.70
pages 349-361

Energy and Antioxidant Status of Rat Liver Mitochondria during Hypoxia- Reoxygenation of Different Duration

Olga A. Gonchar
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Valentina I. Nosar
O.O. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv
Larisa. V. Bratus
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
I. N. Tymchenko
Private Higher Educational Institution "Kyiv Medical University of Ukrainian Academy of Folk Medicine", Kyiv, Ukraine
N. N. Steshenko
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Iryna N. Mankovska
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

ABSTRACT

Changes in the functional activity and protein expression of antiradical Mn-superoxide dismutase (Mn Sod), glutathione-dependent (glutathione peroxidase, glutathione reductase), and NADPH+-generating (isocitrate dehydrogenase) enzymes, as well as the energy metabolism in rat liver mitochondria under hypoxia-reoxygenation of different duration (1, 3, 7, 14 days) were studied. Prolonged hypoxia-reoxygenation was characterized by the phase changes in corticosterone concentration in the rat blood, which corresponded to the changes in energy metabolism and pro and antioxidant balance in rat liver mitochondria. It has been shown that short-term (1day) hypoxia-reoxygenation (5% O2 in the gas mixture) led to an increase in the blood corticosterone concentration and a significant activation of oxidative processes and energy metabolism in rat liver mitochondria, the intensity of which was reduced by the 3rd day. Long-term hypoxia-reoxygenation (7 - 14 days) resulted in gradual depletion of the body adaptive capabilities, as evidenced by a significant decline in blood corticosterone concentration, elevated content of secondary products of lipid peroxidation, imbalance between pro-oxidant and antioxidant reactions, and a reduction of the liver mitochondrial energy capacity. It has been shown that glutathione peroxidase protein expression and enzymatic activity were elevated during the whole experimental period and correlated positively with the level of H2O2. The amount of Mn-SOD protein and enzymatic activity of the latter were lower within first seven days of the experiment and increased in consequent days up to the control level on 14th day. Increased activities of glutathione peroxidase, glutathione reductase, and NADPH+-dependent isocitrate dehydrogenase during prolonged hypoxia-reoxygenation indicate that glutathione and NADPH-generating enzymes are actively involved in the antioxidant protection.