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International Journal of Medicinal Mushrooms

Impact factor: 1.357

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v15.i6.30
pages 539-554

Neurotrophic Properties of the Lion's Mane Medicinal Mushroom, Hericium erinaceus (Higher Basidiomycetes) from Malaysia

Puei-Lene Lai
Mushroom Research Centre, Fungal Biotechnology Lab, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Murali Naidu
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Department of Anatomy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
Vikineswary Sabaratnam
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Kah-Hui Wong
Department of Anatomy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Mushroom Research Centre, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Rosie Pamela David
Department of Anatomy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia
Umah Rani Kuppusamy
Mushroom Research Centre (MRC), Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
Noorlidah Abdullah
Faculty of Science, Mushroom Research Centre & Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia
Sri Nurestri Abdul Malek
Mushroom Research Centre, Fungal Biotechnology Lab.; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia

ABSTRACT

Neurotrophic factors are important in promoting the growth and differentiation of neurons. Nerve growth factor (NGF) is essential for the maintenance of the basal forebrain cholinergic system. Hericenones and erinacines isolated from the medicinal mushroom Hericium erinaceus can induce NGF synthesis in nerve cells. In this study, we evaluated the synergistic interaction between H. erinaceus aqueous extract and exogenous NGF on the neurite outgrowth stimulation of neuroblastoma-glioma cell NG108-15. The neuroprotective effect of the mushroom extract toward oxidative stress was also studied. Aqueous extract of H. erinaceus was shown to be non-cytotoxic to human lung fibroblast MRC-5 and NG108-15 cells. The combination of 10 ng/mL NGF with 1 μg/mL mushroom extract yielded the highest percentage increase of 60.6% neurite outgrowth. The extract contained neuroactive compounds that induced the secretion of extracellular NGF in NG108-15 cells, thereby promoting neurite outgrowth activity. However, the H. erinaceus extract failed to protect NG108-15 cells subjected to oxidative stress when applied in pre-treatment and co-treatment modes. In conclusion, the aqueous extract of H. erinaceus contained neuroactive compounds which induced NGF-synthesis and promoted neurite outgrowth in NG108-15 cells. The extract also enhanced the neurite outgrowth stimulation activity of NGF when applied in combination. The aqueous preparation of H. erinaceus had neurotrophic but not neuroprotective activities.H. erinaceus